Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Clinical Samples in Specialist Hospital, Sokoto

Main Article Content

N. M. Bunza
A. A. Isah
M. D. Hafsat
U. I. Asiya

Abstract

Aim: The study was to determine the antibiotic susceptibility pattern of Staphylococcus aureus isolates against some conventional antibiotics.


Study Design: Hospital based cross-sectional study.


Place and Duration of Study: The study was conducted in Specialist Hospital, Sokoto Metropolis, Sokoto State Nigeria, between June 2018 and September 2018.


Methodology: One hundred (100) Staphylococcus aureus isolates were used in this study. Gram’s staining, catalase, coagulase and mannitol fermentation tests were used to identify and confirm the isolates. Antibiotic susceptibility testing was carried out by disc agar diffusion test.


Results: In the present study 63.0% of the Staphylococcus aureus isolates were from male subjects, while 37.0% were from female subjects. The age group with the highest number of isolates was 11-20 years (37%) and the least (9%) was seen in 41-50 years. Subjects with urine samples had the highest frequency of Staphylococcus aureus isolates with 32.0% and those with high vaginal swab had the lowest 6.0%. The antibiotics susceptibility testing showed that 40% of Staphylococcus aureus isolates were susceptible to Clindamycin, 64% to Ciprofloxacin, 57% to Erythromycin, 71% to Gentamicin, 34% to Cefoxitin, 46% to Quinupristin/Dalfopristin, 58% to Tetracycline and Sulphamethaxazole –Trimethoprim respectively. Screening for MRSA was carried out by antibiotic sensitivity testing using cefoxitin and a prevalence of 66% was obtained. This study showed that Gentamicin and Ciprofloxacin were the most active antibiotics against Staphylococcus aureus.


Conclusion: There is the need for consistent on-going antimicrobial resistance surveillance for important and commonly isolated clinically significant pathogens of staphylococcal species to form the basis for developing and implementing measures that can reduce the burden of antimicrobial resistance and prevent a probable impending public health problem.

Keywords:
Antibiotics, Staphylococcus aureus, MRSA, clinical samples

Article Details

How to Cite
Bunza, N. M., Isah, A. A., Hafsat, M. D., & Asiya, U. I. (2019). Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Clinical Samples in Specialist Hospital, Sokoto. South Asian Journal of Research in Microbiology, 3(3), 1-6. Retrieved from http://journalsajrm.com/index.php/SAJRM/article/view/30085
Section
Original Research Article

References

Yassin NA, Mohammed HH, Ahmad AM. Antibiograming profiles of Staphylococcus aureus isolated from various clinical specimens in Duhok City Iraq. Adv Trop Med Public Health Int. 2013;3(1):25-31.

Jombo GT, Akpan S, Epoke J, Akaa PD, Eyong KI. Methicillin resistant Staphylococcus aureus and their antibiotic sensitivity pattern in Kano, Nigeria. J Med Sci. 2010;1(3):043-046.

Onanuga A, Awhowho GO. Antimicrobial resistance of Staphylococcus aureus strains from patients with urinary tract infections in Yenagoa, Nigeria. J Pharm Bioallied Sci. 2012;4(3):226-30.

Speziale P, Pietrocola G, Provenzano M, Visai L. Structural and functional role of Staphylococcus aureus surface components recognising adhesives matrix molecule of the host. J Nat Med Assoc. 2009;4(2):1337-352.

Karchmer AW. From theory to practice: Resistance in Staphylococcus aureus and new treatments. Clin Microbiol Infect. 2006;12(8):15-21.

Mincey BA, Parkulo MA. Antibiotic prescribing practices in a teaching clinic: Comparison of resident and staff physicians. South Med J. 2001;94(4):365-69.

Todar K. Bacterial resistance to antibiotics. Textbook of bacteriology. 4th Ed. Edinburg: Churchill Livingstone; 2011.

Aboderin OA, Abdu A, Odetoyin BW, Lamikanra AS. Antimicrobial resistance in Escherichia coli strains from urinary tract infections. J Nat Med Assoc. 2009;101(12): 1268-273.

Chessbrough M. District laboratory practice in tropical countries. 2nd Ed. Cambridge: University Press. 2009;62-70:105-114.

Clinical and Laboratory Standard Institute. Performance standards for antimicrobial disc susceptibility tests. 11th Ed. CLSI Document M02-A11. 2012;323-337.

Kumurya AS, Ado ZG. Detection of clindamycin resistance among methicillin resistant Staphylococcus aureus isolates in Kano, Nigerian. Access J Microbiol. 2017;1(2):34-40.

Nwankwo EOK, Abdulhadi S, Magaji A, Ihesiulor G. Methicillin resistant Staphylococcus aureus and their antibiotic sensitivity pattern in Kano, Nigeria. Afr J Clin Exp Microbiol. 2010;11(1):129-36.

Onanuga A. Prevalence and susceptibility pattern of methicillin- resistant Staphylococcus aureus isolates among healthy women in Zaria, Nigeria. Afr J Biotechnol. 2005;4:1321-324.

Odugbemi T. The use and abuse of antibiotics. Nig Med Pract. 1998;1(1):4-8.

Onanuga A, Temedie TC. Nasal carriage of multi-drug resistant Staphylococcus aureus in healthy inhabitants of Amassoma in Niger delta region of Nigeria. Afr Health Sci. 2011;11(2):176-81.

Kumurya AS, Ado ZG. Detection of clindamycin resistance among methicillin resistant Staphylococcus aureus isolates in Kano, Nigerian. Access J Microbiol. 2015;1(2):34-40.

Ikeh EI. Methicillin resistant Staphylococcus aureus at Jos University Teaching Hospital (JUTH). Afr J Clin Exp Microbiol. 2003;4(1):52-55.

Taiwo SS, Bamidele M, Omonigbehin EA, Akinsinde KA, Smith SI, Onile BA, et al. Molecular epidemiology of methicillin resistant Staphylococcus aureus in Ilorin, Nigeria. West Afr J Med. 2005;24(2):100-6.

Onanuga A, Oyi AR, Olayinka BO, Onaolapo JA. Prevalence of community-associated multi-resistant Staphylococcus aureus among healthy women in Abuja, Nigeria. Afr J Biotech. 2005;4(9):942-45.