Anti-aging Activity of Xylaria striata in Drosophila melanogaster

Main Article Content

Shi-Jun Yuan
Wei-Yi Li
Tian-Tian Jian
Yi Huang


Aims: To evaluate the application potential of Xylaria striata Pat. in anti-aging field.

Methodology: Extracting the fruit body of X. striata by ultrasonic-assisted extraction method. In this study, Drosophila melanogaster was used as an anti-aging organism material. After feeding with different concentrations of extract, the natural survival time, survival time under oxidative stress and survival time under UV irradiation of D. melanogaster were all measured. In addition, the in vivo activity of SOD、CAT and MAD, protein concentration and body weight were determined to evaluate the anti-aging effect of ethanol extract from X. striata.

Results: The results showed that the ethanol extract of X. striata could extend the lifespan of D. melanogaster under both irradiation and oxidative stress condition. And the ethanol extract could enhance the activity of CAT in D. melanogaster, especially at concentration of 50 μM, and the content of MAD in D. melanogaster were decreased significantly.

Conclusion: This study clarified the anti-aging activity of X. striata in D. melanogaster and  it would provide some theoretical basis for its further development and utilization in anti-aging drugs and health food.

Xylaria striata, anti-aging, Drosophila melanogaster, lifespan

Article Details

How to Cite
Yuan, S.-J., Li, W.-Y., Jian, T.-T., & Huang, Y. (2019). Anti-aging Activity of Xylaria striata in Drosophila melanogaster. South Asian Journal of Research in Microbiology, 3(3), 1-7. Retrieved from
Original Research Article


Zhao JD. Chinese fungi (The third volume). 1st Ed. Beijing; Science Press;1998.

Sevindik M. Investigation of Antioxidant/Oxidant Status and Antimicrobial Activities of Lentinus tigrinus. Advances in pharmacological sciences. 2018;1718025. DOI:10.1155/2018/1718025

Sevindik M. Investigation of oxidant and antioxidant status of edible mushroom Clavariadelphus truncatus. The Journal of Fungus. 2018; 9(2):165-168.

Gao C, Luo J, Liu X, Ma L, Yuan XH. Recent advances in the studies of chemical compositions and bioactivities of the genus Xylaria. Mycosystema. 2016;35(07):767-781. DOI:10.13346/j.mycosystema.150061

Macias-Rubalcava M, Sanchez-Fernandez RE. Secondary metabolites of endophytic Xylaria species with potential applications in medicine and agriculture. World Journal Of Microbiology & Biotechnology. 2017; 33(01):15.
DOI: 10.1007/s11274-016-2174-5

Song F, Wu SH, Zhai YZ, Xuan QC, Wang T. Secondary Metabolites from the Genus Xylaria and Their Bioactivities. Chemistry & Biodiversity. 2014;11(05):673-694.
DOI: 10.1002/cbdv.201200286

He XS. Images of mushroom fungus in SiChuan. 1st Ed. Bei Jing: Science Press; 2011.

Liu X, Gao C, Zhong YT, Yuan XH, He XS, Ma L. Optimization of submerged culture conditions for Xylaria striata mycelium. Journal of Chinese medicinal materials. 2014; 37(08):1317-21.

Lei CW, Yang ZQ, Zeng YP, Zhou Y, Huang Y, He XS, et al. Xylastriasan A, A new cytochalasan from the fungus Xylaria striata. Natural product research. 2018; 32(01):7-13.
DOI: 10.1080/14786419.2017.1324959

Zhang CJ, Liu X, Zhu P, Liu XL, He XS. Antimicrobial Activities of Ethanol Extracts from Six Species of Higher Fungi against Plant Pathogen. Journal of Anhui Agricultural Sciences. 2013;41(12):5289-5294.

Yuan XH, Zhang CJ, Zheng RL, Xu JR, Liu Z, He XS. Screening of Anti-tumor Activities of Ethanol Extracts from Six Species of Higher Fungi. Journal of Southwest University of Science and Technology. 2013; 28(3):95-97.

Lie CW. Studies on the chemical constituents of Xylaria striata and their bioactivities. Southwest University of Science and Technology; 2017.

Zheng W, Zhang YF, Zhong HQ, Mai SM, Yang XH, Xiang YT. Wuling Capsule for Major Depressive Disorder: A Meta-analysis of Randomised Controlled Trials. East Asian archives of psychiatry : Official journal of the Hong Kong College of Psychiatrists. 2016;26(3): 87-97.

Peng WF, Wang X, Hong Z, Zhu GX, Li BM, Li Z, et al. The anti-depression effect of Xylaria nigripes in patients with epilepsy: A multicenter randomized double-blind study. Seizure-european Journal of Epilepsy. 2015;29:26-33.
DOI: 10.1016/j.seizure.2015.03.014

Hou CL, Sun XL, Tan J, Zhang CX, Yang DS, He XS. Study on Culture of Xylaria striata Fruit Body. Food and Fermentation Sciences & Technology. 2013;49(02):44-46+102.
DOI:1674-506X (2013) 02-0044-0003

Chattopadhyay D, Chitnis A, Talekar A, Mulay P, Makkar M, James J, et al. Hormetic efficacy of rutin to promote longevity in Drosophila melanogaster. Biogerontology. 2017;18(3):397-411.
DOI: 10.1007/s10522-017-9700-1

Proshkina E, Lashmanova E, Dobrovolskaya E, Zemskaya N, Kudryavtseva A, Shaposhnikov M, et al. Geroprotective and Radioprotective Activity of Quercetin, (-)-Epicatechin, and Ibuprofen in Drosophila melanogaster. Frontiers in Pharmacology. 2016;7:505. DOI: 10.3389/fphar.2016.00505

Kaplan EL, Meier P. Nonparametric estimation from incomplete observations. Journal of the American Statistical Association. 1958;53(282):457-481.

Bayliak MM, Lylyk MP, Gospodaryov DV, Kotsyubynsky VO, Butenko NV, Storey KB, et al. Protective effects of alpha-ketoglutarate against aluminum toxicity in Drosophila melanogaster. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 2019;217:40-53. DOI:10.1016/j.cbpc.2018.11.020

Rajpurohit S, Schmidt PS. Latitudinal Pigmentation Variation Contradicts Ultraviolet Radiation Exposure: A Case Study in Tropical Indian Drosophila melanogaster. Frontiers in Physiology. 2019;10:84.
DOI: 10.3389/fphys.2019.00084

Piper MDW, Partridge L. Drosophila as a model for ageing. Biochimica Et Biophysica Acta-Molecular Basis Of Disease. 2018;1864(09):2707-2717.
DOI: 10.1016/j.bbadis.2017.09.016

Niki E. Lipid peroxidation products as oxidative stress biomarkers. Biofactors. 2008;34:171-180.

Li HJ, Zhang SF, Li JX, Zhao Z. Anti-UV Irradiation Effect of Melanin Derived from Apricot Kernel Skin in Drosophila. Food Science. 2012;33(21):285-289.

Jiao SP, Chen B, Du PG. Anti-lipid peroxidation effect of Rosa davuriuca Pall. fruit. Journal of Chinese Integrative Medicine. 2004; 2(5):364-365.

Zhao J, Chen C, Ma N, Sun ZO, Xue WC, Wang H. The Anti-Aging Effect of Phloridein in Male Drosophila melanogaster. Acta Nutrimenta Sinica. 2015;37(04):372-375+383. DOI:10.13325/j.cnki.acta.nutr.sin.2015.04.019

Zhou Q, Zhu L, Zhang D, Li N, Li Q, Dai P, et al. Oxidative Stress-Related Biomarkers in Postmenopausal Osteoporosis: A Systematic Review and Meta-Analyses. Disease Markers. 2016;2016:7067984. DOI: 10.1155/2016/7067984

Lei CW, Yang ZQ, Zeng YP, Zhou Y, Huang Y, He XS, et al. Xylastriasan A, a new cytochalasan from the fungus Xylaria striata. Natural Product Research. 2018; 32(1):7-13.
DOI: 10.1080/14786419.2017.1324959