Isolation of Serratia Marcescens from the Soil and In vitro Prodigiosin Production as Source of Antibiotic, Active against Oxacillin-Resistant Staphylococcus aureus

Main Article Content

B. C. Akin-Osanaiye
I. N. Aruwa
I. W. Olobayotan

Abstract

Background: Wide range of microorganisms produced secondary metabolites as microbial activities in extended habitats.

Aims: The aim of this research is the extraction of the red colored pigment, prodigiosin from Serratia marcesens isolated from the soil and evaluate its antibacterial activity against different strains of oxacillin/methicillin-resistant Staphylococcus S. aureus.

Study Design: Two isolates, namely, RMN1 and RMN2, belonging to the Genus, Serratia from two soil samples collected from two strategic locations in University of Abuja, Nigeria were isolated. The isolates were morphologically distinct on the basis of spore colour, aerial and substrate mycelium formation and production of diffusible pigment. Isolates were Gram stained, observed under a microscope and were seen to be Gram negative. Biochemical tests revealed that the two isolates were catalase and citrate positive, and were oxidase negative. One of the two isolates was observed to have significant antibiotic producing potential, and the antibacterial activity of the produced antibiotics (red pigment extracted from cultural supernatants of the isolates grown on Peptone glycerol agar) was assessed using the agar-well diffusion method and streaking agar method.

Results: The results indicated that the pigment extracted in vitro had varying antibacterial activity at different concentrations of 500 mg/ml, 250 mg/ml, 125 mg/ml and 62.5 mg/ml against four strains of the test organism (Staphylococcus aureus) titled S1 to S4, the extracted pigment was more effective at concentration 500 mg/ml against the antibiotic resistant S. aureus with the MIC at 125 mg/ml.

Conclusion: The results indicated that the soil of this region could be a good source of prodigiosin having antibacterial activity and thus enable the use of micro-organisms as biological control agents.

Keywords:
Isolation, Serratia, in vitro, prodigiosin, antibacterial.

Article Details

How to Cite
Akin-Osanaiye, B. C., Aruwa, I. N., & Olobayotan, I. W. (2019). Isolation of Serratia Marcescens from the Soil and In vitro Prodigiosin Production as Source of Antibiotic, Active against Oxacillin-Resistant Staphylococcus aureus. South Asian Journal of Research in Microbiology, 4(4), 1-9. https://doi.org/10.9734/sajrm/2019/v4i430112
Section
Original Research Article

References

Alanis AJ. Resistance to antibiotics: Are we in the post-antibiotic era? Archives of Medical Research. 2005;36:697-705.

Enright MC. The evolution of a resistant pathogen-the case of MRSA. Current Opinion in Pharmacology. 2003;35:474-479.

Bozdogan B, Esel D, Whitener C. Antibacterial susceptibility of a vancomycin-resistant Staphylococcus aureus strain isolated at the Hershey Medical Center. Journal of Antimicrobial Chemotherapy. 2003;52:864-868.

Chang S, Sievert DM, Hageman JC. Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene. The New England Journal of Medicine. 2003;348:1342-1347.

Wenzel RP. The antibiotic pipeline- challenges, costs, and values. New England Journal of Medicine. 2004;351: 523-526.

Jevitt LA, Smith AJ, Williams PP, Raney PM, McGowan JE, Tenover FC. In vitro activities of daptomycin, linezolid, and quinupristin-dalfopristin against a challenge panel of Staphylococci and Enterococci, including vancomycin-intermediate Staphylococcus aureus and vancomycin- resistant Enterococcus faecium. Microbial Drug Resistance. 2003; 9:389-393.

Meka VG, Gold HS. Antimicrobial resistance to linezolid. Clinical Infectious Diseases. 2004;39:1010-1015.

Nathwani D. Tigecycline: Clinical evidence and formulary positioning. International Journal of Antimicrobial Agents. 2005;25: 185-192.

Dufosse L. Pigments. Microbial Encyclopedia. 2009;4:457-471.

Holt JG, Krieg NR, Sneath PHA, Stanley JT, Williams ST. Bergey’s Manual of Determinative Bacteriology. 9th Edition. Balitimore: Williams and Wilkins, Co; 1994.

Chesebrough M. Medical laboratory manual for tropical countries Volume 11. 2nd Edition, University Press, Cambridge, Great Britain, Pg. 2006;375-380.

Mallikhajuna PB, Prashanthi B, Seetharam YN. In vitro Anthelmintic Activity of Crude Extracts and Alkaloid Fractions from Strychnos potatorum L.f. Pharmacology online. 2010;1:613-617.

Junior A, Zanil C. Biological Screening of Brazilian Medicinal Plants. Brazilian Journal of Science. 2006;95:367-373.

Song MJ, Bae J, Lee DS, Kim CH, Kim JS, Kim SW, Hong SI. Purification and characterization of prodigiosin produced by Integrated Bioreactor from Serratia sp. KH-95. Journal of Bioscience and Bioengineering. 2007;101:157-161.

Bhaswati G, Jayati B. International Journal of Current Microbiology and Applied Sciences. 2014;3(9):169-176.

Jairo L, Hoerlle I, Adriano B. Antimicrobial resistance of Staphylococcus aureus isolated from the intensive care unit of a general hospital in southern Brazil. Journal of Infection in development Countries. 2009;3(7):504-510.

Parani K, Saha BK. Optimization of prodigiosin production from a strain of Serratia marcescens SR1 and screening for antifungal activity. Journal of Biological Control. 2008;22(1):73 79.