Evaluation of Enzymes Production Activity of Trichoderma, Aspergillus and Rhizopus Species in Paraeforce (Herbicide) Degradation

Main Article Content

F. I. Onianwah
H. O. Stanley
V. C. Eze
V. O. Ifeanyi
C. J. Ugboma

Abstract

The study aims to evaluate enzymes that facilitate fungal degradation of paraeforce. Soil samples for fungal isolation were collected from impacted sites and inoculated on potato dextrose agar (PDA). The isolates were screened for growth and tolerance to paraefoce in 50 mg/l concentration of the test herbicides. Trichoderma, Aspergillus and Rhizopus species were found to grow in paraeforce supplemented PDA. Qualitative and quantitative assay for different enzyme production in hydrogen peroxide, methyl red, guaiacol and hydrogen peroxide-pyrogallol complex proved potential for catalase, lignin peroxidase, laccase and manganese peroxidase production, respectively. The results showed that these three fungi have great potential for catalase, peroxidase and laccase production after six days aerobic incubation in paraeforce and these enzymes facilitated the utilization of the paraeforce.  

Keywords:
Soil, enzymes, fungal degradation, paraeforce.

Article Details

How to Cite
Onianwah, F. I., Stanley, H. O., Eze, V. C., Ifeanyi, V. O., & Ugboma, C. J. (2020). Evaluation of Enzymes Production Activity of Trichoderma, Aspergillus and Rhizopus Species in Paraeforce (Herbicide) Degradation. South Asian Journal of Research in Microbiology, 5(3), 1-7. https://doi.org/10.9734/sajrm/2019/v5i330131
Section
Original Research Article

References

Baran N, Mouvet C, Negrel P. Hydrodynamic and geochemical cons-traints on pesticide concentrations in the groundwater of anagricultural catchment (Brevilles, France). Environmental Pollu-tion. 2007;148(3):729-738.

Belal EB, Zidan NA, Mahmoud HA, Eissa FI. Bioremediation of pesticides–con-taminated soils. J. Agric. Res. Kafr-El-sheikh. Univ. 2008;34:588–608.

Castillo JM, Casas J, Romero E. Isolation of an endosulfan degrading bacterium from a coffee farm soil: Persistence and inhibitory effect on its biological functions. Sci. Total Environ. 2011;5:412-413.

Baldwin BC, Bray MF, Goeghegan MJ. The microbial decomposition of paraquat, biochem. Journal. Syngenta File no. PP 148/0546.1977;101(2):15.

Aneja KR. Experiments in microbiology, plant pathology and biotechnology. 4th Edn. New Age International Pvt, Ltd, India; 2005.

Barnett HL, Hunter B. Illustrated genera of imperfecti fungi, 3rd edn. Burges publish-ing company, USA; 1972.

Shivani D, Jain SK. Extracellular enzy-matic profile of fungal deteriogens of historical palace of Ujjain. Int. J. Current Microbiology. 2005;4:122-132.

Caridis KA, Christakopulos P, Macris BJ. Simultaneous production of glucose oxidase and catalase by Alternaria alternata. Appl. Microb. Biotechnol. 1991; 34:794–797.

Kiiskinen LL, Ratto M, Kruus KJ. Fungal production of extracellular laccase. Appl. Microbiol. 2004;97:640-646.

Jhadav A, Vamsi KK, Khairnar, Y. Fungal enzymes production. Int. J. Microbiol. Res. 2009;1:9-12.

Adiveppa B, Basawanneppa K. Production and optimization of laccase by submerged fermentation. Current Research. 2015;7 (7):18308.

Lopez MJ, Guisando G, Vargas-Garcia, MC, Suarez-Estrella F, Moreno J. Degradation of industrial dyes by lignolytic microorganisms isolated from composting environment. Enzymes Microbial Technol. 2006;40:42-45.

Archibald FS. A new assay for lignin-type peroxidase employing the dye Azure B, Applied and Environmental Microbiology. 1992;58:3110–3116.

Rayner AD, Boddy LW. Fungal decomposition of wood. Its biology and ecology. Wiley NY; 1988.

Paszczynski A, Cnawford RL, Huynh VB. Manganese Peroxidase of Phanerochaete chrysosporium: Purification, Methods in Enzymology. 1988;264-270.

Subhani A, EI-ghamry AM, Huang C, Xu J. Effect of pesticides (Herbicides) on soil microbial biomass: A rewiew. Pakistan Journal of Biological Sciences. 2000;3(5): 705-709.

Kanayama N, Suzuki T, Kawai K. Purification and characterization of an alkaline manganese peroxidase from Aspergillus terreus LD-1. Journal of Bioscience and Bioengineering. 2002;93: 405–410.