Characterization and Application of Aspergillus tubingensis USMI03 RPf10 Fermented Solution as a Nutrient Source for Microbial Growth

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Shimaa A. Amin
Hemmat M. Abdelhady
Khadiga A. Abou-Taleb
Zahra H. Tayeb
Rania F. Ahmed


The fermented solution of this strain contains the highest concentration of citric acid, indole acetic acid (IAA) and elements as well as phosphatase activity. Using the Aspergillus tubingensis USMI03 RPf10 fermented solution as a medium led to decrease in the growth rate of tested bacterial isolates (Bacillus subtilis, Pseudomonas fluorescens and Escherichia coli) and fungal isolates (Aspergillus sp. and Penicillium sp.) except Trichoderma viride which gave the same growth on fermented solution treatments compared to control. Whereas the tested yeast isolates (Candida olivera and Saccharomyces cerevisiae) recorded the same growth rate on fermented solution treatments as whole media and higher growth rate on fermented solution + glucose treatment, compared to control. Also, the latter treatment resulting in higher growth of some tested bacteria (B. subtilis and P. fluorescens) and the same growth rate of other bacteria (E. coli), compared to synthetic media. The growth of Rhizopus sp. decreased on fermented solution treatments than control. It is suggested that, using A. tubingensis USMI03 RPf10 fermented solution as a whole medium or as a mineral could be used as a source for microbial growth which varied from one microorganism to another.

Aspergillus tubingensis, biosolubilization, rock phosphate fermented solution, microorganisms, microbial growth improvement

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How to Cite
Amin, S. A., Abdelhady, H. M., Abou-Taleb, K. A., Tayeb, Z. H., & Ahmed, R. F. (2020). Characterization and Application of Aspergillus tubingensis USMI03 RPf10 Fermented Solution as a Nutrient Source for Microbial Growth. South Asian Journal of Research in Microbiology, 6(2), 1-9.
Original Research Article


Khan MS, Zaidi A, Ahemad M, Oves M, Wani PA. Plant growth promotion by phosphate solubilizing fungi – current perspective. Arch. Agron. Soil Sci. 2010; 56:73–98.

Sahu SN, Jana BB. Enhancement of the fertilizer value of rock phosphate engineered through phosphate-solubilizing bacteria. Ecol. Eng. 2000;15:27-39.

Yadav BK, Tarafdar JC. Phytase activity in the rhizosphere of crops, trees and grasses under arid environment. J. Arid. Environ. 2004;58:285–293.

Yadav BK, Tarafdar JC. Ability of Emericella rugulosa to mobilize unavailable P compounds during Pearl millet [Pennisetum glaucum (L.) R. Br.] crop under arid condition. Indian J. Microbiol. 2007;47:57–63.

Yadav BK, Tarafdar JC. Availability of unavailable phosphate compounds as a phosphorus source for clusterbean (Cyamopsis tetragonoloba (L.) Taub.) through the activity of phosphatase and phytase produced by actinomycetes. J. Arid. Legum. 2007;4:110–116.

Achal V. Solubilization of phosphate rocks and minerals by wild type and UV induced mutants of Aspergillus tubingensis. M.Sc. thesis submitted to Thapar Institute of Engineering and Technology University of Biotechnology and Environmental Sciences, Patiala; 2005.

Aseri GK, Jain N, Tarafdar JC. Hydrolysis of organic phosphate forms by phosphatases and phytase producing fungi of arid and semi-arid soils of India. American-Eurasian J. Agric. Environ. Sci. 2009;5(4):564-570.

Pradhan N, Sukla LB. Solubilization of inorganic phosphates by fungi isolated from agriculture soil. Afr. J. Biotechnol. 2005;5:850-854.

Vyas P, Gulati A. Organic acid production in vitro and plant growth promotion in maize under controlled environment by phosphate-solubilizing fluorescent Pseudomonas. BMC. Microbiol. 2009;9:74.

Mendes GO, Dias CS, Silva IR, Juúior JIR, Pereira OL, Costa MD. Fungal rock phosphate solubilization using sugarcane bagasse. World J. Microbiol. Biotechnol. 2013;29:43-50.

Scervino JM, Mesa MP, Mónica ID, Recchi M, Moreno NS, Godeas A. Soil fungal isolates produce different organic acid patterns involved in phosphate salts solubilization. Biol. Fertil Soil. 2010;46:755-763.

Jain R, Saxena J, Sharma V. Solubilization of inorganic phosphates by Aspergillus awamori S19 isolated from rhizosphere soil of a semi-arid region. Ann. Microbiol. 2012; 62:725-735.

Sheshadri S, Ignacimuthu S. Effect of nitrogen and carbon sources on the inorganic phosphate solubilization by different Aspergillus niger strains. Chem. Eng. Comm. 2004;191:1043-1052.

Reddy MS, Kumar S, Babita K, Reddy MS. Biosolubilization of poorly soluble rock phosphates by Aspergillus tubingensis and Aspergillus niger. Bioresour. Technol. 2002;84:187-189.

Oliveira CA, Alves VMC, Marriel IE, Gomes EA, Scotti MR, Carneiro NP, Guimarães CT, Schaffert RE, Sá NMH. Phosphate solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian Cerrado Biome. Soil Biol. Biochem. 2008; 41:1782-1787.

Abdelhady HM, Abou-Taleb KA, Ali SA, Abd el-salam SS, Tayeb ZH. Effect of carbon and nitrogen sources on phosphate solubilization by some local isolates from Egyptian rock phosphate deposit. Res. J. Pharm. Biol. Chem. Sci. 2016;8:452-469.

Abou-Taleb KA, Amin SA, Abdelhady HM, Tayeb ZH. Optimization of rock phosphate solubilization in submerged cultures containing some agro-industrial residues. J. Adv. Microbiol. 2018;11:1-18.

EMCC, Egyptian Microbial Culture Collection. Microbiological Resource Center, Fac. of Agric., Ain Shams Univ. Cairo-Mircen, Egypt. 1992;126.

Thom C, Church MB. The Aspergilli. Baltimore, Maryland, USA: Williams & Wilkins; 1926.

Difco Manual. Dehydrated culture media and reagents for microbiology. Laboratories incorporated Detroit. Michigan, 48232 USS; 1984.

Wickerham LJ. Taxonomy of yeasts. Tech. Bull. U.S. Dept. Agric. 1951;1029:1-55.

Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1956;28:350-356.

Glickmann E, Dessaux Y. A critical examination of the specificity of the Salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Appl. Environ. Microb. 1995;61:793-796.

Marier JR, Boulet M. Direct determination of citric acid in milk with improved pyridine-acetic anhydride method. J. Dairy Sci. 1958;41:1683-1692.

Hillman G. Continuous photometric measurement of prostrate acid phosphatase activity. Z. Klin. Chem. Klin. Biochem. 1971;9:273-274.

Painter PR, Marr AG. Mathematics of microbial populations. Annual Rev. Microbiol. 1963;22:219-221.

Doelle HW. Bacterial metabolism. 2nd (Edn). Academic Press, New York; 1975.

Vassileva M, Vassilev N, Azcon R. Rock phosphate solubilization by Aspergillus niger on olive cake based medium and its further application in a soil plant system. World J. Microbiol. Biotechnol. 1998;14: 281-284.

Yadav J, Verma JP, Tiwari KN. Plant growth promoting activities of fungi and their effect on chickpea plant growth. Asian J. Biolog. Sci. 2011;4:291-299.

Alam S, Khalil S, Ayub N, Rashid M.. In vitro solubilization of inorganic phosphate by phosphate solubilizing microorganisms (PSM) from maize rhizozphere. Int. J. Agr. Biol. 2002;1560-8530.

Padmavathi T. Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology. J. Soil Sci. Plant Nutr. 2015;15:781-79.

Nopparat C, Jatupornipat M, Rittiboon A. Isolation of phosphate solubilizing fungi in soil from Kanchanaburi, Thailand. KMITL Sci. Tech. J. 2007;7:137-146.

Tahir A, Mateen B, Saeed S Uslu, H. Studies on the production of commercially important phytase from Aspergillus niger ST-6 isolated from decay inorganic soil. Micol. Aplicada. Int. 2010;22:51–57.

Jena SK, Rath CC. Effect of environmental and nutritional conditions on phosphatase activity of Aspergillus awamori. Curr. Res. Environ. Appl. Mycol. 2014;4:45–56.

Jena SK, Rath CC. Optimization of culture conditions of phosphate solubilizing activity of bacterial sp. isolated from similipal biosphere reserve in solid-state cultivation by response surface methodology. Int. J. Curr. Microbiol. Appl. Sci. 2013;2:47–59.

Leveau JHJ, Lindow SE. Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290. Appl. Environ. Microbiol. 2005;71: 2365-2371.

Nasim G, Rahman M. Cytokinin priming as a tool to induce in vitro growth and biomass production of some soil fungi. Pak. J. Bot. 2009;41:1445-1452.

Calvo AM, Wilson RA, Bok JW, Keller NP. Relationship between secondary metabolism and fungal development. Microbiol. Mol. Biol. Rev. 2002;66:447–459.