Antimicrobial Activities of Allium sativum on Microorganisms Isolated from Spoilt Tomatoes Sold in Awka Anambra State, Nigeria

Oledibe, Odira Johnson *

Department of Botany, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

Ejimofor, Chiamaka Frances

Biological Science Department, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.

Afam-Ezeaku, Chikaodili Eziamaka

Department of Botany, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

Nwakoby, Nnamdi Enoch

Microbiology Department, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.

Mbaukwu, Onyinye Ann

Department of Botany, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

Onuzulike, Mariagoretti Chinecherem

Department of Botany, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

*Author to whom correspondence should be addressed.


Tomato is a universally consumed vegetable crop. Its spoilage involves changes in which they become less palatable, less attractive to the eyes or even toxic to consumers. These changes may be accompanied by alterations in taste, smell, appearance or texture. The aim of this study is to identify the spoilage pathogens of tomatoes and to ascertain the antimicrobial potential of garlic ethanol extract on the isolates. Spoilt tomato samples were purchased from three markets (Eke Awka, Nnamdi Azikiwe temporary site (Temp. site) and Amenyi) in Awka, Anambra State. They were transported Alpha Laboratory, Awka in a sterile polythene bags for microbial isolation and analysis. The media used for the isolation were Nutrient Agar and Sabouraud Dextrose Agar, both were placed into a conical flask autoclaved at 121ºC at 15psi for 20 minutes and the plates were incubated at room temperature. The bacteria isolated from the tomato fruits were: Pseudomonas sp, Bacillus sp, Staphylococcus sp and Escherichia coli while the fungi isolates were Aspergillus sp, Penicillium sp and Mucor sp. The antimicrobial activity of ethanol garlic extract against these microorganisms isolated from spoilt tomatoes were determined using disc method. Three different concentrations (25%, 50%, 100%) of ethanol garlic extract were used to test for the antimicrobial activity. The result showed that at different concentrations the ethanol garlic extract possess antimicrobial properties on the selected organisms apart from Pseudomonas sp and Penicillium sp where there was no zone of inhibition. The presence of these microorganisms is hazardous to health. Therefore, garlic extract could be used as a beneficial substitute of synthetic chemicals.

Keywords: Tomatoes, microorganism, garlic, antimicrobial activity

How to Cite

Johnson, O. O., Frances, E. C., Eziamaka, A.-E. C., Enoch, N. N., Ann, M. O., & Chinecherem, O. M. (2022). Antimicrobial Activities of Allium sativum on Microorganisms Isolated from Spoilt Tomatoes Sold in Awka Anambra State, Nigeria. South Asian Journal of Research in Microbiology, 14(1-2), 46–58.


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Arbrar S, Abera D, Simegne K, Ali M. Effect of storage conditions and packing materials on shelf life of tomato. Food Sci Qual Manag. 2016;56:2224-6088.

Hosea ZY, Liamngee K, Owoicho AL, David T. Effect of Neem leaf powder on postharvest shelf life and quality of tomato fruits in storage. International Journal of Development and Sustainability. 2017; 6(10):1334-49.

Mohammad A, Abdullahi A, Nasiru AM. Effects of neem leaves and garlic bulbs on fungi causing post-harvest spoilage of tomatoes in Sokoto North Western Nigeria. J Agric Vet Sci. 2021;14(16):8-14.

Eno-Abasi S, Gbenga A, Joke M, Abdulganiu A. A long way to self-sufficient in tomato production. Sunday Mag. 2018; 50:7-8.

Gustavsson J, Cederberg C, Sonesson U, Vanotterdijk R, Meybeck A. Global food losses and food waste- Extent, Causes and Prevention; 2011.


Accessed 2nd January 2022.

Irokaanulo EO, Egbezien IL, Owa SO. Uses of Moringa oleifera in the preservation of fresh tomatoes. J Agric Vet Sci. 2015;8(2):127-32.

Enyiukwu DN, Awurum AN, Nwaneri JA. Efficacy of plant derived pesticides in the control of myco induced postharvest rots of tubers and agricultural products. Net J Agric Sci. 2014;2(1):30-46.

Neeta NP, Gol B, Ramana R. Effect of post-harvest treatment on physicochemical characteristic of and shelf life of tomato fruits during storage. Am Eurasian J Agric Environ Sci. 2010;9:470-9.

Agwaranze DI, Kalu AC, Sabo IA, Aso RE, Okachi MA. Mycologicalassessment of deteriorated tomato fruits sold in Wukari, Nigeria. J Biotechnol Res. 2020;6(7):84-9.

Akinyele BJ, Akinkunmi CO. Fungi associated with the spoilage of berry and their reaction to magnetic field, Microbiology. Biotechnol Food Sci. 2012; 2(2):701-12.

Nicosia D, Pangallo S, Raphael G, Pomeo F, Strano M, Rapisard P, Droby S, Schena L. Control of postharvest fungal rots on citrus fruit and sweet cherries using a pomegranate peel extract. Postharvest Biology and Technology. 2016;114:54-61.

James CJ, Natalie S. Microbiology. A Laboratory Manual. 2013;43.

Abasiubong G, V, Amadi C. Ikon. Adv Microbiol. Antimicrobial activity of garlic extract On organisms isolated from tomato rot. 2017;7(2):1-10.

Wogu M, Ofuase O. Microorganisms responsible for the spoilage of tomato fruits sold in markets in Benin City Southern Nigeria. Sch Acad J Biosci. 2014;2(7): 459-66.

Ogundipe FO, Bamidele FA, Adebayo-Oyetoro AO, Ogundipe OO, Tajudeen OK. Incidence of bacteria with potencial pubic health impications on raw Tomato sold in Lagos State, Nigeria. Niger Food J. 2012; 30(2):106-13.

Liamgee K, Iheanacho A, Aloho K. Isolation, identification and pathogenicity of fungi causing postharvest spoilage of tomato fruits during storage. Annu Res Rev Biol. 2018;26(6):1-7.

Mohammad D, Kuhiyep C. Bacteria and fungi co-biodeterioration of selected fresh tomatoes sold within Kaduna. Sci World J. 2020;15(1):48 55.

Shang A, Cao SY, Xu XY, Gan RY, Tang GY, Corke H et al. Bioactive compounds and biological functions of garlic (Allium sativum L.). Foods. 2019;8(7):246.

Mugao LG. In-vitro activity of selected plant extract on post-harvest pathogens causing tomato fruit rot. J Phytopharmacol. 2021;10(4):236-41.

Tijjani A, Adebitan S, Gurama A, Aliyul M, Haruna S, Mohammad G et al. In vitro and in vivo efficacy of some plant extracts for the control of tomato fruit rot caused by Aspergillus flavus; 2014.

Ashraf Z, Ali Q, Rashid M, Malik A. Antibacterial, antifungi and anticancer activities of multistress affected Allium sativum plant extracts. Plant Cell Biotechnol Mol Biol. 2020;21:55 13 22.

Saravanasingh K, Frdrik G, Ramamurthy M. A study on antibacteria and antifungal activities of extract of medicinal plant Aegle marmelos. Int J Adv Res Biol Sci. 2016; 3(2):321-8.

Ben Senhaji B, H, Salghi R. Asteriscus imbricatus extract: antifungal activity and anticorrosion inhibition. Int J Electrochem Sci. 2013;8:6033-46.

Wagner L, Stielow JB, de Hoog GS, Bensch K, Schwartze VU, Voigt K et al. A new species concept for the clinically relevant Mucor circinelloides complex. Persoonia. 2020;44:67-97.

Abramson D, Lombaert G, Clear RM, Sholberg P, Trelka R, Rosin E. Production of patulin and citrinin by Penicillium expansum from British Columbia (Canada) apples. Mycotoxin Res. 2009;25(2): 85-8.

Oliveira D, Borges A, Simões M. Staphylococcus toxins and their molecular activity in infectious diseases. Toxins. 2018;10(6):30-8.

Bitrus A, Peter O, Abbas M, Goni M. Staphylococcus: A review of antimicrobial resistance mechanisms. Veterinary sciences research and reviews. 2018;4:2-10.

World Health Organization. Escherichia coli; 2018

[Cited Feb 2, 2022].


Chaudhuri RR, Henderson IR. The evolution of E. coli phylogeny. Infect Genet Evol. 2012;12(2):214-26.

Silby MW, Winstanley C, Godfrey SA, Levy SB, Jackson RW. Pseudomonas genomes: diverse and adaptable. FEMS Microbiol Rev. 2011;35(4):652-80.

Breidenstein EB, de la Fuente-Núñez C, Hancock RE. Pseudomonas: all roads lead to resistance. Trends Microbiol. 2011; 19(8):419-26.

Barbier F, Andremont A, Wolff M, Bouadma L. Hospital acquired pneumonia and ventilator associated pneumonia, recent advances in epidemiology and management. Curr Opin Pulm Med. 2013; 19(3):216-28.