Preliminary Screening for Antibacterial Properties of the Male Flowers of Phoenix dactylifera

Main Article Content

Emad M. Abdallah

Abstract

Objective: The aim of this study was to evaluate the antibacterial potential of the male flowers of Phoenix dactylifera (date palm tree) against five Gram-positive and five Gram-negative bacteria.

Methods: Male flowers were collected and extracted by maceration using 80% methanol and the antibacterial activity was determined using cup-plate diffusion test, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests.

Results: The methanol extract of male flowers of Phoenix dactylifera showed varying degrees of antibacterial activity against tested bacterial strains, the most susceptible Gram-positive bacteria were Bacillus cereus and Streptococcus pneumonia which recorded 12.2±0.3 and 9.0±0.0 mm zone of inhibition (ZI), MIC values were 50 and 100 mg/ml, MBC values were 200 and ˂200 mg/ml, respectively. The most susceptible Gram-negative bacteria were Proteus vulgaris, Klebsiella pneumonia and Pseudomonas aeruginosa which recorded 10.0±0.0, 9.7±0.3 and 9.0±0.0 mm ZI, MIC values were 100 mg/ml and MBC values were 200 mg/ml, respectively.  Based on MBC/MIC ratio, the extract has some degree of bactericidal effect. However, the results were not competitive with the standard drug (Chloramphenicol).

Conclusion: As a result, the tested methanol extract of male flowers of date palm tree exhibited some degree of antibacterial activity with a bactericidal property. More future studies such as fractionation process are required to isolate and investigate its bioactive compounds.

Keywords:
Antibacterial activity, agar-well diffusion, MIC, MBC, Phoenix dactylifera.

Article Details

How to Cite
Abdallah, E. M. (2019). Preliminary Screening for Antibacterial Properties of the Male Flowers of Phoenix dactylifera. South Asian Journal of Research in Microbiology, 3(2), 1-6. https://doi.org/10.9734/sajrm/2019/v3i230081
Section
Original Research Article