Microbiological and Nutritional Changes Associated with the Thermal Processing and Fermentation of African Oilbean (Pentaclethra macrophylla) Seeds for ugba Production
South Asian Journal of Research in Microbiology,
Boiled slices of African oil bean (Pentaclethra macrophylla) seeds were subjected to a 72-hour traditional fermentation to produce ugba for the evaluation of the effect of boiling and fermentation on the nutritional status of the seeds. Bacterial isolates recovered from the fermenting substrate include Bacillus, Streptococcus, Salmonella, Escherichia coli, Pseudomonas, Staphylococcus, Klebsiella, Micrococcus, Lactobacillus and Proteus species while Saccharomyces cerevisiae, Penicillium, Aspergillus, Fusarium and Rhizopus spp were the fungi isolated. Bacillus and Lactobacillus spp were the predominant bacterial starter cultures because they persisted from the beginning till the end of the fermentation. Saccharomyces cerevisiae was the predominant fungus. Fermentation and boiling improved the nutritional values of the protein, fat, fibre, moisture and ash contents of the ugba samples after 72 hours of fermentation with an increase in pH value towards alkalinity. However, both processes decreased the carbohydrate content of the ugba. Sensory evaluation showed that the laboratory-produced ugba sample compared with the local market sourced ugba in sensory properties. At the end of the fermentation, food borne spoilers and pathogens that were part of the contaminats of the fermenting samples were eliminated by the microbial metabolites thus making ugba safe for human consumption. The ugba can also stay for some days without spoilage in the absence of refrigeration.
- nutritional status
- oil beanseed
How to Cite
Okafor JC, Okolonkwo UC, Ejiofor MAN. Evaluation of oils from fermentedand unfermented seeds of the African oil bean tree pentaclethra macrophylla. International Tree Crops Journal. 1991;7:95-101.
Mbajunwa OK, Akingbala JO, Mulongoy K, Oguntimein G. Starter CultureEvaluation for the Production of ugba from African oil bean seed (Pentaclethra macrophylla).Jjournal of Sci.ence Food. Agric.ulture. 199877: 127- 132.
Njoku HO, Okemadu C. Biochemical changes during the natural fementations of the African oil bean (Penthaclethra Macropylla) for the production of ugba. Journal of Science in Food Agriculture. 1989;49:457-465.
Enujiugha VN, Agbede JO. Nutritional and anti-nutritional characteristics of African oil bean (Pentaclethra macrophylla benth). Seeds. Application in Tropical Agriculture. 2000;5:11-15.
Enujiugha VN. Nutrient changes during the fermentation of African oil bean (Penttoclethra macrophylla benth) seeds. Pakistan Journal of Nutrition. 2003;2:320–323.
Achinewhu SC. The effect of fermentation on carbohydrate and fatty acidComposition of the African oil bean (Pentaclethra macrophylla) seed. Food Chemistry. 1986;19:105–116.
Enujiugha and Olagundoye. Chemical composition, functional and sensory properties of maize-based snack (Elekute) enriched with African oil bean seed (Pentaclethra macrophylla benth) African Journal of Food Science. 2001;10(12): 379-384.
Enujiugha VN, JO Agbede JO. Nutritional and anti-nutritional characteristics of African oil bean (Pentaclethra macrophylla Benth) seeds. Application of Tropical. Agricuture. 2000;5:11-14.
Obeta J. A note on the (Pentaclethra macrophylla). Journal of Applied Bacteriology. 1983;54:433-433.
Odunfa SA, Oyeyiola GF. Microbiological study of the fermentation of ugba, a Nigerian indigenous fermented food flavour. Journal on Plant Foods. 1985;6: 155-163.
Mbata T, Orji MU. Process optimization in the production and Preservation of ugba, A Nigerian fermented food. International. Journal of Microbiology. 2008;4:2-6.
Enujiugha VN, Akanbi CT. Quality evaluation of canned fermented African oil bean seed slices during ambient storage. African Journal Food Science. 2008;2:54–59.
Dhakal S, Aryal P, Aryal S, Bashyal D, Khadka D. Phytochemical and antioxidant studies of methanol and chloroform extract from leaves of Azadirachta indica A. Juss. in Tropical region of Nepal. Journal of Pharmacognosy and Phytotherapy. 2016;8(12):203-208.
Rachael and Oluwamodupe. Effect of fermentation on nutritional and anti-nutritional properties of fermenting Soy beans and the antagonistic effect of the fermenting organism on selected pathogens. Int. Res. J. Microbiol. 2012;3(10):333-338.
Cowan CL, Steel KJ. Manual of identification of medical bacteria. 3rd edition. Cambridge University Press, London, UK; 1974.
Agbulu CO, Gberikon GM, Ajine BO. Isolation and characterization of microorganisms associated with second hand female undergarments and children wear sold in Makurdi Metropolis Int. J. Curr. Microbiol. App. Sci. 2015;4(1):716-724
Cheesbrough M. District laboratory practical in tropical countries part 2. Cambridge University Press London. 2006; 28-39.
AOAC. Official method of analysis. 18th editon. Association of Official Analytical Chemist, Washington DC; 2010.
Eze VC, Onwuakor CE, Ukeka E. Proximate composition, biochemical and microbiological changes associated with fermenting African oil bean (Pentaclethra macrophylla Benth) seeds. American J. Journal of Microbiology. 2014;2:674–681.
Fogarty WM, Griffin PJ, Joyce AM. Enzymes of bacillus species-part 1. Process Biochemistry. 1974;11–24.
Isu NR, Njoku HO. An evaluation of the microflora associated with fermented African oil bean (Pentaclethra macrophylla Benth) seeds during ugba production. Plant Foods for Human Nutrition. 1997;51: 145-157.
Olasupo NA, Okorie CP, Oguntoyinbo FA. The biotechnology of ugba, a Nigerian Traditional Fermented Food Condiment. Frontiers in Microbiology. 2016;7:1153.
Onwuakor C, Nwaugo VO, Nnadi CJ, Emetole JM. Effect of varied culture conditions on crude supernatant (Bacteriocin) production from four lactobacillus species isolated from locally fermented maize (Ogi). American Journal of Microbiological Research. 2014;2(5): 125-130.
Odunfa SA, Oyewole OB. Identification of bacillus species from iru, fermented African locust bean product. J. Basic Microbiol. 1986;26:101–108.
Daeschel F, Torriani S. Physiological characters and distribution of Lactic acid bacteria isolated from maize silage. J. Appl. Bacteriol. 1987;60:83-92.
Oguoke CC, Aririatu LE. Microbial and organoleptic changes associated with ugba stored at ambient temperature. Nigeria. Food Journal. 2004;22:133-140.
Jideani IAO, Okereke CR. Comparative studies of microorganisms and sensory attributes of condiments from the fermentation of different seeds. Plant food Human Nutrition. 1991;41:27-34.
Mulyowidarso RK, Fleet GH, Buckle KA. Association of bacteria with the fungal fermentation of soybean tempe. Journal of Applied Bacteriology. 1990;68:43-47.
Achi OK. Microorganisms associated with natural fermentation of Prosopis africana seeds for the production of okpehe. Plant. Foods Human Nutrition. 1992;42:304–309.
Alinnor IJ, Oze R. Chemical evaluation of nutritive value of Pentaclethra macrophylla Benth (African oilbean) seeds. Pakistan Journal of Nutrition. 2011;10(4):355-359.
Ikhuoria U. Esther, Anthony E. Aiwonegbe, Peace Okoli and Macdonald Idu. Characteristics and composition of African oil bean seed (Pentaclethra macrophylla Benth). Journal of Applied Sciences. 2008; 8:1337-1339.
Oseni A, Ekpeigin M. Studies on biochemical changes in maize wastes fermented with Aspergillus niger. Biokemistri. 2007;19(2):75-79.
Oladele EOP, Oshodi AA. Effect of fermentation on some chemical and nutritive properties of Berlandier nettle Spurge (Jatropha cathartica) and physic nut (Jatropha curcas) seeds. Pakistan Journal of Nutrition. 2008;7:292-296.
Oseni OA, Akindahunsi AA. Some phytochemical properties of the effect of fermentation on the seed of Jatropha Curcas L. American Journal of Food Technology. 2011;6:158-165.
Jonathan SG, Ajayi I, Omitade Y. Nutritional compositions, fungi and aflatoxins detection in stored ‘gbodo’ fermented (Dioscorea rotundata) and ‘elubo ogede’ fermented (Musa parasidiaca) from south western Nigeria. African Journal of Food Science. 2011;5(2):105-110.
Augustin J, Johnson SR, Teitzel C, Toma RB, Shaw RL, True RH, Hogan JM. Vitamin composition of freshly harvested and stored potatoes. Journal of Food Science. 1978;43(5):1566-1570.
Norman NP, Joseph HH. Food science. 5th edn., Chapman and Hall Publishers, New York. 1995;55.
Omafuvbe BO, Olumuyiwa SF, Osuntogun BA, Adewusi SRA. Chemical and biochemical changes in African locust bean (Parkia biglobosa) and melon (Citrullus vulgaris) seeds during fermentation to condiments. Pak J Nutr. 2004;3(3):140–145.
Aderibigbe EY, Odunfa SA. Growth and extra cellular enzyme production by strains of bacillus species isolated from fermenting African locust bean, iru. Journal of Application in Bacteriology. 1990;69:662-671.
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