Prevalence and Antibiotic Sensitivity Patterns of Salmonella enterica serovar typhimurium Isolated from Local Cheese in Ondo State, Nigeria

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Anthony Kayode Onifade
Oladapo Daniel Omololu


Aims: To determine the prevalence and current sensitivity status of Salmonella enterica serovar typhimurium. Typhimurium to commercial antibiotics in cheese at the Southern Western part of Nigeria.

Study Design: Experimental design.

Place and Duration of Study: Cheese samples were bought from nomad cheese vendors across various suburbs in three major districts (Akure, Ikare and Owo) of Ondo state.

Methodology: A total of two hundred and sixty (260) cheese samples were vended from various suburbs across the aforementioned senatorial districts of Ondo State. Isolation of Salmonella enterica serovar typhimurium. Typhimurium from cheese samples were carried out on Salmonella-shigella (S.S) and were identified biochemically through standard microbiological techniques. The identified strains were subjected to ten (10) conventional antibiotics for their sensitivity patterns.

Results: The distribution patterns of the S. enterica serovar typhimurium. Typhimurium isolated were 9(47.4%), 8(42.10%) and 2(10.5%) in Ikare, Akure, and Owo respectively. The degree of resistance of the Salmonella enterica serovar typhimurium. Typhimurium to the various antibiotics used was 6.3% to Chloramphenicol (30 µg); 6.3% to Ofloxacin (10 µg); 6.3% of Sparfloxacin (10 µg); 12.5% to Streptomycin (30 µg); 18.8% to Sceptrin; 18.8% to Ciprofloxacin (10 µg); 25% to Gentamycin (10 µg); 31.3% to Amoxicillin (30 µg); 37.5% to Pefloxacin (10 µg) and 56.3% to Augmentin (30 µg).

Conclusion: Cheese creates a favourable environment for a variety of microorganisms and can be important source of foodborne pathogens.

Salmonella enterica serovar typhimurium, cheese, antibiotics, multidrug resistant

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How to Cite
Onifade, A. K., & Omololu, O. D. (2020). Prevalence and Antibiotic Sensitivity Patterns of Salmonella enterica serovar typhimurium Isolated from Local Cheese in Ondo State, Nigeria. South Asian Journal of Research in Microbiology, 5(3), 1-10.
Original Research Article


Amiri S, Moradli GH. Molecular identification of virulence genes (agfA and mgtC) in Salmonella Typhimurium strains isolated from children with gastroenteritis using multiplex PCR method. J Babol Uni Med Sci. 2016;18(10):40-45.

Jessica LF, David G, Ludovic V, Nicholas H, Christine H, Derek P, Subhankar M, Gordon D. The interaction of Salmonella enterica serovar Typhimurium with intestinal organoids derived from human induced pluripotent stem cells. Infect Immun. 2015;00161-15.

Larrisa B, Moonjely S, Behie SW, Bidochka MJ. Fungi with multifunctional lifestyles: Endophytic insect pathogenic fungi. Plant M. Biol. 2016;90(6):657-664.

USDA/ARS (U.S. Department of Agriculture/Agricultural Research Service). National Nutrient Database for Standard Reference Release 27; 2011.
[Accessed January 5, 2015]

Ogbolu DO, Terry AAO, Oluremi AS, Olanrewaju AA. Microbial contamination of locally produced cheese and determination of their antimicrobial potential in Nigeria. Afri. J. Clin. Exper. Microbiol. 2014;15(2): 76-83.

Sandle T, Satyada R. Determination of the cleaning efficiency for glassware in the pharmaceutical microbiology laboratory. Eur J Parent Pharma Sci. 2016;21(1):16-22.

Cheesbrough M. District laboratory practice in tropical countries. 2nd Ed., Cambridge University Press, Cambridge, UK. 2006;50:75-77.

McHugh AJ, Feehily C, Hill C, Cotter PD. Detection and enumeration of spore-forming bacteria in powdered dairy products. Front. Microbiol. 2017;8:109.

Abhishek V, Kumari N, Saina P, Menghani E. Isolation, identification and characterization of oil-degrading bacteria isolated from the contaminated sites of Barmer, Rajasthan. Int J Biotechnol Bioeng Res. 2013;4(5):429-436.

CLSI. Performance standards for antimicrobial disk susceptibility tests. 13th Edition. CLSI Standard M02. Wayne, PA: Clinical and Laboratory Standards Institute; 2018.

Beshiru AT, Igbinosa EO, Igbinosa IH. Prevalence of antimicrobial resistance and virulence gene elements of Salmonella serovars from ready-to-eat (RTE) shrimps. Front. Microbiol. 2019;10:1613.

Frasson I, Bettanello S, De Canale E, Richter SN, Palù G. Serotype epidemiology and multidrug resistance patterns of Salmonella enterica infecting humans in Italy. Gut Pathog. 2016;8(1): 26.

Scuderi G, Fantasia M, Filetici E, Anastasio MP. Foodborne outbreaks caused by Salmonella in Italy, 1991–4. Epidemiol Infect. 1996;116(3):257-265.

Budiati T, Rusul G, Wan-Abdullah WN, Arip YM, Ahmad R, Thong KL. Prevalence, antibiotic resistance and plasmid profiling of Salmonella in catfish (Clarias gariepinus) and tilapia (Tilapia mossambica) obtained from wet markets and ponds in Malaysia. Aquaculture. 2013;372:127-132.

Carvalho FC, Sousa OV, Carvalho EM, Hofer E, Vieira RH. Antibiotic resistance of Salmonella spp. isolated from shrimp farming freshwater environment in Northeast Region of Brazil. J. Pathog; 2013.
DOI: 10.1155/2013/685193