Effect of Endophytic Bacteria Burkholderia cepacia on Growth, Cocoon Characters and Enzyme Activity of Silkworm, Bombyx mori L.

Main Article Content

V. Gunasekhar
Arpitha Somayaji


Microorganisms have always been of scientific prominence and their indulgence in industrial and research facets cannot be denied. Several microorganisms have been employed as research tool to amend various parameters of industrial prominence. The current research article is from the context of significance emphasizing on the silk industry. The research article has focused on the impact of Burkholderia cepacia which is an endophytic bacterium on the silk worm and the affirmative aspects were recorded. The research work included the inoculation of the bacterium with the silk worm which has resulted in enhanced production of silk from the inoculated lot. In addition, the research has also demonstrated higher activity of protease and amylase in inoculated lot when compared to control population. The results obtained have substantiated the practical aspect of the research.

Burkholderia cepacia, Bombyx mori, endophytic bacteria, microorgan, silk worm.

Article Details

How to Cite
Gunasekhar, V., & Somayaji, A. (2020). Effect of Endophytic Bacteria Burkholderia cepacia on Growth, Cocoon Characters and Enzyme Activity of Silkworm, Bombyx mori L. South Asian Journal of Research in Microbiology, 5(4), 1-8. https://doi.org/10.9734/sajrm/2019/v5i430134
Original Research Article


Yasuhiko Matsumoto, Kazuhisa Sekimizu. Silk worm as an experimental animal for research on fungal infections. Microbiology and Immunology. 2019;63:2.

Masitta Tanjung, Maryani Cyccu Tobing, Darma Bakti, SyafruddinIlyas. Isolation of total RNA silkworm larvae C301 on their several body tissues. International Journal of Scientific and Technology Research. 2016;5:2277-8616.

Yanhua Yang, Hanhan Tang, Yuanyuan Zhang, Feifei Zhu, Peng Lü, Qin Yao, Keping Chen. Research progresses on immune mechanism of silk worm Bombyx mori. Physiological Entamology. 2018;43(3).

Krishnasamy S, Sundaramullary TS. Sericulture manual 2: Silkworm rearing; 1991.

Fang Lu, Zhaoyuan Wei, Yongjiang Luo, Hailong Guo, Guoqing Zhang, Qingyou Xia, Yi Wang. Silk: Visualising and exploring multiple data of silk worm. Nucleic acid Research. 2020;48(3):D749-D755.

Nagaraju J. Application of genetic principles for improving silk production. Currentscience. 2002;83:409-414.

Aleksey Chenko NA, Galanova AV, Ananyev PP, Nadkernichnaya EV, Gonchar YA. The increase of productivity of mulberry plantations as a result of using bacterial fertilizers. Sericologia. 2004;44: 387-392.

Prasad NR. Silkworm disease management and its limitations. Indian Silk. 1999;31:7-9.

Sannapa B, Ramesh MJ, Chandrappa D. Influence of castor genotype on consumption indices of Eri silkworm sumia Cynthia ricini. Bioduval Environ Eco. 2002;20:960-964.

Sashidran Nair K, Nair JS, Trivedy K, Vijayan VA, NirmalKumar S. Efficacy of feed conversation of the last instar silkworm Bombyx mori L. under the influence of a juvenoid, R394. Indian Journal of Sericulture. 2004;43:187-193.

Xianling Ji, Guobing Lu, Yingping Gai, Huijv Gao, Baoyun Lu, Lingrang Kong, Zhimei Mu. Colonization of Morus alba by the plant growth promoting and antagonistic bacterium Burkholderia cepacia strain Lu. 2010;10-1(10):243.

Rao RDM, Kodandaramaiha J, Reddy MP, Katiyar RS, Rahamuthalla VK. Effect of VAM fungi and bacterial biofertilizers on mulberry leaf quality and cocoon characters under semiarid conditions. Caspian Journal of Environmental Sciences. 2007;5(2):111-117.

Thirumalaisamy R, Gowrishankar J, Suganthapriya S, Prakash B, Ashokkumar L, Arunachalam G. Genetic variability in Morus alba L. by biochemical and bioassay methods for increased silk productivity. Journal of Biomed Science and Research. 2009;1:11-18.

Dillon RJ, Vennard CT, Keith Charnley A. Exploitation of gut bacteria in thelocust. Nature. 2000;403:851.

Sekar P, Balasundaram A, George John. A study on the establishment of bacterial microbiotain the gut of silkworm Bombyx mori. International Journal of Current Research. 2010;11:192-199.

Singh KK, Chauhan RM, Pande AB, Gokhale SB, Hegde NG. Effect of use of Lactobacillus plantarum as a probiotics to improve cocoon production of mulberry silkworm Bombyx mori (L). Journal of Basic and Applied Sciences. 2005;1:1-8.

Subramanian S, Mohanraj P, Sivakumar S, Muthuswami M. Influence of antibiotics and probiotics on endogenous gut micro flora in silkworm Bombyx mori. Sericologia. 2010;50:333-342.

Esaivani C, Vsanthi K, Bharthi R, Chairman K. Impact of probiotic Saccharomyces cerevisiae on the enzymatic profile and the economic parameters of silkworm Bombyx mori L. Open Access Scientific Publisher. 2014;1:1-7.

Sengupta K, Singh BD, Mustafi JC. Nutrition of silkworm Bombyx mori L. studies on enrichment of mulberry with various sugars, proteins, amino acids and vitamins for vigorous growth of worms and increased cocoon production. Indian Journal Sericulture. 1972;11:11-19.

Vitthalrao B, Khyadeand Rajendra M, Marathe. Diversity of bacterial flora in the midgut of fifth instar larvae of silkworm, Bombyx mori (Race: PM×CSR2). G.J.B.B. 2012;1:191-200.

Mohanraj P, Subramanian S. Antibacterial activity of gut flora isolates from mulberry silkworm Bombyx mori. An international Quarterly Journal of Environmental Sciences (Specialissue). 2014;1:267-270.

Dadd RH. Digestion in insect in: Chemicalzoology. Academic Press New York. 1970;5:117-145.

Sunder Raj S, Chinnaswamy KP, Neelu Nangia. Soyabeanto Boost Cocoon Production Indian Silk. 2000;11-13.

Plummer T. David. An introduction to practical biochemistry. Tata McGraw-Hill Publishing Company Ltd. 1988;184-186.

Mohamed Sadiq A, Govindaraju K, Singaravelu G. UV impact on the digestive physiology of Bombyx mori L. Journal of Biopesticides. 2008;1:226-228.