The Antibacterial Efficacy of Annona muricata (Linn) Leaves against Some Enteric Bacteria

Main Article Content

Kehinde Yemisi Akinde
Ebenezer Oluyemi Dada

Abstract

Aims: This research was carried out to evaluate the susceptibility patterns of some enteric bacteria to aqueous, ethanol and methanol extracts of Annona muricata leaf.

Study Design: Experimental design.

Place and Duration of Study: Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria. Between January, 2019 and May, 2019.

Methodology: Extraction of bioactive components of leaves was done by maceration and phytochemical screening was carried out on the leaf extracts to determine the bioactive components present. The bacteria isolates were subjected to antibiotic sensitivity test using standard methods while the  antibacterial  activity  of  the  plant  extracts on human enteric bacteria was  determined  using  agar well dilution method. A. muricata bark extracts were purified using column chromatography method. The minimum inhibitory and minimum bactericidal concentrations (MIC/MBC) of the extracts was performed using tube dilution technique.

Results: The phytochemical screening of leaf extracts showed presence of saponins, tannins, flavonoids, terpenoid and glycosides. Antibacterial activities of the leaf extracts revealed that aqueous, ethanol and methanol extracts had highest zone of inhibition of 22.00±0.58 mm, 24.67±0.33 mm and 24.00±0.58 mm respectively on Salmonella typhi. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) for ethanol extract ranged between 25-100 mg/ml and 50-200 mg/ml respectively while methanol extract ranged between 25-50mg/ml and 50-100 mg/ml respectively. Ciprofloxacin (CPX) was significantly (P≤0.5) the most active of all the antibiotics used against the test organisms (both clinical and typed isolates).

Conclusion: This research revealed that A. muricata leaf extracts possesses antibacterial activity against human enteric bacteria isolates used in this study. The commercial antibiotics were observed to be effective in inhibiting the test organisms. The purified extracts of A. muricata leaf showed higher inhibitory effect compared to crude extracts which can serve as ingredient for the production of novel antibacterial agents for the treatment of infections caused by enteric bacteria.

Keywords:
Annona muricata leaf, phytochemical constituents, antibacterial activity, food borne diseases, enteric bacteria

Article Details

How to Cite
Akinde, K. Y., & Dada, E. O. (2020). The Antibacterial Efficacy of Annona muricata (Linn) Leaves against Some Enteric Bacteria. South Asian Journal of Research in Microbiology, 7(1), 19-29. https://doi.org/10.9734/sajrm/2020/v7i130161
Section
Original Research Article

References

McMahon M. What is Enteric Bacteria?

(Accessed March 1 2014)

Available:http://www.wisegeek.com/

Nas F, Ali M, Yahaya A. Identification of some enteric bacteria from diarrhoeic stool samples of patients attending Murtala Muhammad Specialist Hospital Kano, Nigeria. International Journal of Advanced Academic Research Sciences, Technology & Engineering. 2017;3(9):28-35.

World Health Organization. WHO estimates of the global burden of foodborne diseases: Foodborne disease burden epidemiology reference group 2007-2015.

Thomas MK, Murray R, Flockhart L, Pintar K, Pollari F, Fazil A, Nesbitt A, Marshall B. Estimates of the burden of foodborne illness in Canada for 30 specified pathogens and unspecified agents, Circa 2006, Foodborne Pathogens and Disease. 2013;10(7).

Faleye OS, Dada EO. Effects of Ethanol Extract of Unripe Annona muricata (l.) Fruits on the Haematological and Histopathological Parameters in Swiss Albino Rats Infected with Salmonella typhi. British Journal of Pharmaceutical Research. 2016;9(1):1-13.

Bandiola TMB, Ignacio GB, Yunson EGA, Bandiola PDB. Int. J. Applied. Pharmaceutical. Bio. Res. 2017;2(6):15-23.

Sejal P, Jayvadan KP. A review on a miracle fruits of Annona muricata. Journal of Pharmacognosy and Phytochemistry. 2016;5(1):137-148.

Mishra S, Ahmad S, Kumar N, Sharma BK. Annona muricata (the cancer killer): A review. Global Journal Pharmaceutical Res. 2013;2:1613–1618.

Abdul Wahab SM, Jantan I, Haque MA, Arshad L. Exploring the leaves of Annona muricata L. as a source of potential anti-inflammatory and anticancer agents. Frontiers in Pharmacology. 2018;9(661):1-20.

Kadir HA, Moghadamtousi SZ, Fadaeinasab M, Nikzad S, Mohan G, Ali HM. Annona muricata (Annonaceae): A review of its traditional uses, isolated acetogenins and biological activities. International Journal Molecule Science. 2015;16:15625-15658.

Chukwuka KS, Ikheloa JO, Okonko I0, Moody JO, Mankinde TA. Advances in Applied Science Research. 2011;2(4): 37-4.

Atta HM, Dabur SM, Desoukey SG. Sparsomycin antibiotic production by Streptomyces sp. AZ-NIOFDI: Taxonomy, fermentation, purification and biological activities. Amenrican-Eurasian Journal of Agricultural and Evironmental Science. 2009;5(3):368-377.

Association of official analytical chemist. Laboratory manual on basic methods in analytical chemistry education consults. 26th edition, In: Official methods of analysis; 2011.

Cheesbrough M. District laboratory practice in Tropical countries.2nd edition, Cambridge University Press, UK; 2006.

Doughari J, Pukuma M, De N. Antibacterial effects of Balanites aegyptiaca L. Drel. and Moringa oleifera Lam. on Salmonella typhi, African Journal of Biotechnology. 2007;6 (19):2212-2215.

Edet UO, George UE, Mboto CI, Mbim EN, Umego CF, Sunday H. Comparative evaluation of the effect of Annona muricata (Graviola) leaves extracts and cefoxitin on Staphylococcus aureus. Asian Journal of Biology. 2017;3(3):1-7.

Dada EO, Akinde KY. Susceptibility patterns of some enteric bacteria to crude and purified bark extracts of Annona muricata L. Journal of Advances in Medical and Pharmaceutical Sciences. 2020;22(2): 21-33.

Muhammad A, Auwal U, Olanitola SO, Lawan FD. Antibacterial activity of fractionated extracts of Carica papaya leaves and stem bark against clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA). Modern Application in Pharmacy and Pharmacology. 2018;1(5):1-5.

Leke L, Onaji RA, Galadima A, Okoronkwo MU. Phytochemical screening and antimicrobial activity studies of the root extract of Anthocleista djalonensis (Cabbage Tree). International Journal of Chemistry. 2012;4(4):37.

Adamu A, Edeghagba B, Olatomi F, Ezeokoli O, Elijah A. Microorganisms associated with commercial motor cycle helmets in Lagos metropolis. Journal of Microbiology, Biotechnology and Food Sciences. 2012;1(15):1179-1188.

Ogundare AO, Akinyemi AI. Antibacterial properties of the leaf extracts of Anthocleista djalonensis A. Chev on some pathogenic organisms. Journal of Medicinal Plants. 2014;4(1):75-85.

Kuta FA, Oyedum U, Garba SA, Bala JD, Adedeji SA. Antibacterial activity of Vitellaria paradoxa on some enteric bacteria. Nigerian Journal of Microbiology. 2017;31(1):3882-3892.

Mailard JY. Bacterial target sites for biocide action. Journal of Applied Microbiology. 2002;9:16-27.