Molluscicidal Assessment of Aqueous Extract of Moringa oleifera Lam Seed on Bulinus Snail for the Control of Schistosomiasis

Main Article Content

Umetiti Chukwuemeka Nnamdi
Ene Paschal Chuka
Orji Nkeiru Mary Ann
Umedum Chinelo U.
Ugwu Kenneth Chukwudi
Ikeanumba Michael Okwudiri

Abstract

Aims: This research is essentially on the Molluscicidal assessment of Aqueous extract of Moringa oleifera Lam seed on Bulinus Snail for the control of Schistosomiasis.

Study Design: This is a controlled study where a total of 810 Bulinus snails were collected from three different streams with each stream representing a community from each of the three senatorial zones of Anambra state where schistosomiasis was endemic. Aqueous extract of Moringa oleifera (Lam) seed at different concentrations were used on the Bulinus snails to determine their molluscidal properties.

Place and Duration of Study: Two hundred and Seventy (270) Bulinus snails were collected from streams representing each of the three senatorial zones of Anambra state viz; Obutu Lake, Omogho town, Orumba North Local Government Area (Anambra South), Agulu Lake, Agulu town, Aniocha Local Government Area (Anambra Central) and Omambra River, Omor town, Ayamelum Local Government Area (Anambra North) This research was carried out between November 2018 to April 2019.

Methodology: Aqueous dilutions of the grinded Moringa oleifera (Lam) seed were exposed to the Bulinus snails from the various streams for 24 hours, after which the snails were removed from the experimental test solution and washed thoroughly with dechlorinated tap water and transferred to containers with fresh dechlorinated tap water for another 24 hours of recovery. The snails were incubated at 28 ± 5°C and fed with lettuce leaves.

Results: Aqueous Moringa oleifera Lam seed extract was toxic to Bulinus adult snails in a dose dependent manner and the total Lethal Concentration (LC50 and LC90) values determined after 24 hours exposure from the whole streams were 468 ppm and 813 ppm respectively.

Conclusion: Aqueous seed extract of Moringa oleifera Lam was toxic to Bulinus snail even at low concentrations.

Keywords:
Bulinus, Moringa oleifera, schistosomiasis, LC50, LC90, streams, Anambra State

Article Details

How to Cite
Nnamdi, U. C., Chuka, E. P., Mary Ann, O. N., Chinelo U., U., Chukwudi, U. K., & Okwudiri, I. M. (2020). Molluscicidal Assessment of Aqueous Extract of Moringa oleifera Lam Seed on Bulinus Snail for the Control of Schistosomiasis. South Asian Journal of Research in Microbiology, 7(1), 30-39. https://doi.org/10.9734/sajrm/2020/v7i130162
Section
Original Research Article

References

Kiros G, Erko B, Mekonnen Y. Laboratory assessment of molluscicidal and cercariacidal effects of Glinus lotoides fruits. Res Notes. 2014;7:1- 22.

Rizk MZ, Aly HF. Recent therapeutic approaches in control of parasitic diseases with special reference to schistosomiasis. Int J Adv Res. 2015;1:957–971.

Ekwunife CA, Ukaga CN, Okafor FC. Urinary schistosomiasis in Anambra State, Nigeria. Nig J Parasitolo. 2005;25:127-131.

World Health Organization. The control of Schistosomiasis. World Health Organization Technical Report Series. 2017;830:34 – 39.

Ekpo F, Laja-Deile A, Oluwole S, et al. Urinary schistosomiasis among pre-school children in rural community near Abeokuta, Nigeria. Para & Vect. 2010;3:58-68.

Nwoke BEB, Dozie INS, Nwoke JC, Amosike JC. Human schistosomiasis and Nigerian environment and climate change. Bio-Res. 2004;2(1):103-114.

Kane RA, Stothard JR Emery AM, et al. Molecular characterization of freshwater snails in the genus Bulinus: A role for barcodes? Para & Vect. 2008;1:15.

Salem HK, Omram NE, Eissam SH, et al. Induction of teratogenesis of freshwater snail (Biomphalaria alexandrina) using the molluscicide niclosamide. Afr J Sci. 2014;2:255-268.

World Health Organization. The control of schistosomiasis. Report of a WHO Expert Committee. World Health Organization Technical Report Series. 2014;728:65-67.

Yang F, Long E, Wen J, et al. Linalool, derived from Cinnamomum camphora leaf extracts, possesses molluscicidal activity against Oncomelania hupensis and inhibits infection of Schistosoma japonicum. Para & Vect. 2014;7:40-47.

Rawani A, Ghosh A, Chandra G. Laboratory evaluation of molluscicidal & mosquito larvicidal activities of leaves of Solanum nigrum. Ind J. Med Res. 2014;140:285–295.

Choy SY, Prasad KM, Wu TY. Utilization of plant-based natural coagulants as future alternatives towards sustainable water clarification. J Env Sci. 2014;26:2178–2189.

Araújo LC, Aguiar JS, Napoleão TH. Evaluation of cytotoxic and anti-inflammatory activities of extracts and lectins from Moringa oleifera seeds. PLoS One. 2013;8:81973.

Kayode RM, Afolayan AJ. Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds. J Zhejiang Uni Sci. 2015;16:680–689.

Santos AF, Argolo AC, Coelho L, et al. Detection of water soluble lectin and antioxidant component from Moringa oleifera seeds. Water Res. 2005;39:975–980.

Santos AF, Luz LA, Argolo AC, et al. Isolation of a seed coagulant Moringa oleifera lectin. Process in Biochem. 2009; 44:504–508.

Weis WI, Drickamer K. Structural basis of lectin-carbohydrate recognition. Ann Rev Biochem. 1996;65:441–473.

Ferreira RS, Napoleão TH, Santos AF. Coagulant and antibacterial activities of the water-soluble seed lectin from Moringa oleifera. Letter in App Microbiol. 2011;53: 186–192.

De-Lima-Santos ND, de-Moura KS, Napoleão TH. Oviposition-stimulant and ovicidal activities of Moringa oleifera lectin on Aedes aegypti. PLoS One. 2012;7: 44840

De-Oliveira CF, Luz LA, Paiva PM.Evaluation of seed coagulant Moringa oleifera lectin as a bio-insecticidal tool with potential for the control of insects. Proc Biochem. 2011;46:498–504.

Silva CL, Vargas,TS, Baptista D, et al. Molluscicidal activity of Moringa oleifera on Biomphalaria glabrata: Integrated dynamics to the control of the snail host of Schistosoma mansoni. Rev Brasil Farmaco. 2013;23:848–850.

Ibrahim AM, Abdalla AM. Impact of Moringa oleifera seed aqueous extract on some biological, biochemical and histological aspects of Biomphalaria alexandrina snails. Env Sci Poll Res. 2017; 24(36):28072–28078.

Salawu O, Odaibo AB. The molluscicidal effects of Hyptis suaveolens on different stages of Bulinus globosus in the laboratory. African J Biotech. 2011;10: 10241–10247.

World Health Organization. Report of scientific working group on plant molluscicide and guidelines for evaluation of plant molluscicide, Bulletin of World Health Organization. 1980;4:83.

Finney DJ. Probit Analisys. 3rd ed. Cambridge University Press, New Deli. 1971;24–32.

Bakry FA. Use of some plant extracts to control Biomphalaria alexandrina snails with emphasis on some biological effects. Pest Biochem & Physio. 2009;95:159–165.

Mohamed AM, El-Emam MA, Osman, GY. Effect of Basudin, Selecron and the phytoalkaloid Colchicine (pesticides) on biological and molecular parameters of Biomphalaria alexandrina snails. Pest Biochem & Physio. 2012;102:68–78.

Pinto CL, Silva AC, Tatiana SV, et al. Molluscicidal activity of Moringa oleifera on Biomphalaria glabrata: integrated dynamics to the control of the snail host of Schistosoma mansoni. Braz J Pharmacognosy. 2013;23:848-850.

Daya LD, Chothani JM, Vaghasiya VU. Assessment of the effects of Balanites aegyptiaca mesocarp and aqueous extract of Moringa oleifera leaves on Bulinus truncatus and cercariae of Schistosoma heamatobium. Pharmacogn Rev. 2015;72: 55–62.

Ferreira PMP, Carvalh, AFU, Farias DF. Larvicidal activity of the water extract of Moringa oleifera seeds against Aedes aegypti and its toxicity upon laboratory animals. J Egy Soc Parasitol. 2009;81: 207–216.

Rocha-Filho CA, Albuquerque LP, Silva LR. Assessment of toxicity of Moringa oleifera flower extract to Biomphalaria glabrata, Schistosoma mansoni and Artemia salina. Chemosphere. 2015;45: 188–192.