Effects of Ethanol Extract of Bridelia ferruginea (W.) on the Haematological and Histopathological Parameters in Swiss Albino Rats Infected with Salmonella typhi

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Busayo Temitope Akinyele
Ebenezer Oluyemi Dada


Aims: To study the effects of ethanol stem bark extract of Bridelia ferruginea on the hematological and histopathological parameters in Swiss albino rats infected with Salmonella typhi.

Study Design: Experimental design.

Place and Duration of Study: Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria. Between January, 2018 and June, 2018.

Methodology: Fresh stem bark of Bridelia ferruginea was collected, dried, powered, and extracted using 70% ethanol. Twenty – seven rats of the same age between 90 -120 g in weight were selected and divided into 9 groups containing three each. The infectivity dose (ID) was determined with the clinical S. typhi. The dose of the B. ferruginea stem bark extract (50-5000 mg/kg) used in this study was administered orally for 7 days. At the end of the treatment period, the rats fasted overnight. Then blood samples were collected by cardiac puncture for haematological studies and thereafter sacrificed. Organs (liver and kidney) were excised for relative organ weight analysis and histopathological studies.

Results: The infectious dose (ID) of S. typhi on experimental rats in this study was 3.2x106cfu/ml. The weights of liver and kidney in all groups under observation slightly increases (treated with ciprofloxacin and ethanol extract) when compared to the control(uninfected) except for the infected/treated where the weight was significantly reduced. However, The Packed Cell Volume (PCV) and Haemoglobin (Hb) of rats treated with a higher dose of the ethanol (5000mg/ml) extract increased significantly (p<0.05) when compared to the control(uninfected) while the infected but not treated reduced significantly(p<0.05) when compared with the uninfected, infected and treated with Ciprofloxacin and extract groups. The infected and untreated group has the highest reduction in red blood cell counts(RBC) with 3.40 g/l respectively which were significantly different at p  when compared to other treatment groups while a non-significant decrease was observed in the Red Blood Cell (RBC) of rats administered with various concentrations of ethanol extract and ciprofloxacin after treatment. White Blood Cells (WBC) of rats given the extract significantly reduced when compared with the control (un-infected) group while there was no significant difference in the lymphocyte count of rat administered the extract of B. ferruginea and ciprofloxacin. Neutrophils of rats in all groups significantly increased (p<0.05). However, the monocyte and eosinophil level were not significantly different from the control. There was dilation of sinusoids depletion separating the hepatic cord in place lined by kupffer cells and not necrosis with possible deposition of immunological materials observed in the liver of rats infected/treated with the extract at various concentrations. Similarly, there were loss of bowman capsule, severe karyolysis and several tubular drainages with possible deposition of immunological materials in the glomeruli basement of the kidney after treatment with the extract of B. ferruginea

Conclusion: The stem bark ethanol extract of Bridelia ferruginea can be found relatively safe. However, excessive use might be toxic to the to the kidney and the liver.

Bridelia ferruginea, Salmonella typhi, haematological, histopathological, rats

Article Details

How to Cite
Akinyele, B. T., & Dada, E. O. (2020). Effects of Ethanol Extract of Bridelia ferruginea (W.) on the Haematological and Histopathological Parameters in Swiss Albino Rats Infected with Salmonella typhi. South Asian Journal of Research in Microbiology, 7(2), 1-18. https://doi.org/10.9734/sajrm/2020/v7i230165
Original Research Article


Awodele O, Agbaje EO, Ogunkeye FA, Kolapo AG, Awodele DF. Towards integrating traditional medicine (TM) into National Health Care Scheme (NHCS): Assessment of TM practitioners’ disposition in Lagos, Nigeria. Journal of Herbal Medicine. 2011;1:90–94.

Farnsworth NR, Soejarto DD. Potential consequences of plant extinction in the United States on the current and future availability of prescription drugs. Econ Bot.1985;39(3):231–240.

Bakoma B, Eklu-Gadegbeku K, Berké BA, Agbonon A, Aklikokou K, Gbeassor M. Chemical components and effect on streptozotocin-induced diabetes of Bridelia ferruginea benth root bark ethyl acetate fraction. International Journal of Pharma and Bio Sciences. 2015;6(1):843- 853.

Faleye OS, Dada EO. Effects of ethanol extract of unripe Annona muricata (l.) fruits onthehaematological and histopathological parameters in Swiss albino rats infected with Salmonella typhi. British Journal of Pharmaceutical Research. 2016;9(1):1-13. 2.

Burkil HM. The Useful Plants of West Tropical Africa. The Royal Botanic Garden, Kew.1994;2:636.

Adeleye AO, Oloyede OI, Bolarinwa TO, Adebayo OA. Inhibitory effects of aqueous extract of Bridelia ferruginea Stem Bark on Iron (II) Sulphate - Induced Oxidative Stress in Brain and Liver of Albino Wistar Rats. Journal of Applied Life Sciences International. 2017;14(2):1-7.

Ahn K. The worldwide trend of using botanical drugs and strategies for development global drugs. BMB Reports. 2017;50(3):111–116.

Kwesi Prah Thomford, Ronald Yeboah, AmaKyeraa Thomford, Dominic AdoteyEdoh, Merlin Lincoln Kwao Mensah, Alfred Ampomah Appiah. A Retrospective clinical study on the effectiveness of the aqueous leaf extract of Bridelia ferrugenia(Benth) in the Management of Diabetes Mellitus. International Journal of Herbal Medicine. 2015;2(5):43-45.

OloyedeOl, Babalola SO. In-vitro Antioxidant activity of ethanolic extract of Bridelia ferruginea. International Journal of Academic Research. 2(3):2223-2234.

Alba S, Bakker MI, Hatta M, Scheelbeek PFD, Dwiyanti R, Usman Ret al. Risk factors of typhoid infection in the Indonesian archipelago. PLoS One. 2016;11:13(3):1-9.

Darton TC, Zhou L, Blohmke CJ, Jones C, Waddington CS, Baker S, et al. Blood culture- PCR to optimise typhoid fever diagnosis after controlled human infection identifies frequent asymptomatic cases and evidence of primary bacteraemia. J Infect. 2017;74:358–366.

Dahiya S, Sharma P, Kumari B, Pandey S, Malik R, Manral N, et al. Characterization ofntimicrobial resistance in Salmonellae during 2014–2015 from four centres across IndiaAn ICMR antimicrobial resistance surveillance network report. Indian J Med Microbiol.2017;35:61.

Patel D, Desai S, Devkar R, Ramachandran AV. Acute and sub-chronic toxicological evaluation of hydromethanolic extract of Coriandrum sativum L. Seeds. EXCLI Journal. 2012;11:566575.

Cheesbrough M. District laboratory practice in Tropical countries. 2nd edition, Cambridge University Press, UK. 2014;434.

Dada EO, Komolafe OI. In vivo evaluation of the inhibitory effects of Ocimum gratissimum on Salmonella typhi. Intl. J. Pharm. & Biol. Res. 2013;4(4):185-191.

Reddy YRR, Lokanatha O, Ratnam KSVP, Reddy CS, Naga Raju I, Reddy CD. Acute and sub acute toxicity of Moringa oleifera stem bark extract in Swiss albino mice. Intl. J. Life Sci. Biotechnol. & Pharma Res. 2013;2(4):73-84.

Larbie C, Arthur FKN, Woode E, Terlabi EO. Evaluation of acute and subchronic toxicity of A. muricata (Linn) aqueous extract in animals. Euro. J. Exp. Bio. 2011;1(4):115-124.

Jothy SL, Zakaria Z, Chen Y, Lau YL, Latha LY, Sasidharan S. Acute oral toxicity of methanolic seed extract of Cassia fistula in Mice. Molecules. 2011;16:5268-5282.

OlufunshoAwodele, Kennedy IliyaAmagon, Joseph Agbo MNV Prasad. Toxicological evaluation of the aqueous stem bark extract of Bridelia ferruginea (Euphorbiaceae) in rodents. Interdiscip Toxicol. 2015;8(2):89–98.

DOI: 10.1515/intox-2015-0014

Awe EO, Banjoko SO. Biochemical and haematological assessment of toxic effects of the leaf ethanol extract of Petroselinum crispum (Mill) Nyman ex A.W. Hill (Parsley) in rats. BMC Complem. &Altern. Med. 2013;13:75-80.

Odesanmi SO, Lawal RA, Ojokuku SA. Haematological effects of ethanolic fruit extract of Tetrapleura tetraptera in male Dutch witch rabbits.Res. J. Med. Plants. 2010;4(4):213-217.

Ejere VC, Nnamonu EI, Chukwuka CO, Ugwu GC, Ejim AO, Asogwa CN. Effect of aqueous extract of Hibiscus sabdariffa calyces on haematological characteristics of Rattusnovergicus. Anim. Res. Intl. 2013; 10(3):1809-1816.

Isaac LJ, Abah G, Akpan B, EkaettelU. Haematological properties of different breeds anssexs of rabbits(p.24-27). Proceedings of the 18thAnnual Conference of Animal Science Association of Nigeria.

Soetan KO, Akinrinde AS, Ajibade TO. Perliminarystudies on the haematological parameters of cockerels fed raw and processed guineacorn(Sorghum bicolor) (p.49-52). Proceedings of 38th Annual Conference of Nigerian Society for Animal Production.

Omodamiro OD, Nwankwo CI. The effects of Voacanga africana leaf extract on serum lipid profile and haematological parameters on albino wistar rats. Euro. J. Exp. Biol. 2013;3(3):140-148.

Willey JM, Sherwood LM, Woolverton CJ. Microbiology. 7th edition. McGraw Hill Publisher, New York; 2008.

McKnight DC, Mills RG, Bray JJ, Crag PA. Human Physiology. 1999;4th Edition. PP. 290–294, Churchill Livingstone.

Owoseni AA, AyanbamijiTA,Ajayi YO, Ewegbenro IB. Antimicrobial and phytochemical analysis of leaves and bark extracts from Bridelia ferruginea. Afr J Biotechnol. 2010;9(7):1031–1036.

Ezejindu DN, Udemezue OO, Akingboye AJ. Protective effects of Moringaoleifera leaf extract on the kidneys of adult wistar rats. Am. J. Eng. Res. 2014;3(2): 157-161.

Harizal SN, Mansor SM, Hasnan J, Tharakan JKJ, Abdullah J. Acute toxicity study of the standardized methanolic extract of Mitragyna speciose Korthin Rodent. J. Ethnopharm.2010;131:404409.

Bakoma BB, Berke B, Eklu-Gadegbeku K, Agbonon A, Aklikokou K, Gbeassor M, Creppy EE, Moore N. Acute and sub-chronic (28 days) oral toxicity evaluation of hydroethanolic extract of Bridelia ferruginea Benth root bark in male rodent animals. Food Chem Toxicol. 2013;52: 176–179.