Main Article Content
Aim: To determine the bacterial and physicochemical analysis of topsoil from an electronic waste (e-waste) dumpsite within Port Harcourt metropolis using standard procedures.
Place and Duration of Study: The study area was Kaduna street, beside Fruit and Vegetable Garden Market, Mile l which is located in Port Harcourt, Rivers State, Nigeria. The coordinates are 4o47’57.5” N 7o00’02.7” E. The duration of study was between March and September, 2019.
Methodology: The waste disposed were mainly television sets, computer monitors, radio sets, stoves, laptops and central processing units. Soil samples were cleared off top debris, collected within 5cm of the top soil from four (4) different points of the dumpsite and a control was collected from area devoid of waste disposal, 20m away from the dumpsite. The five samples were kept in clean sterile polythene bags. Contamination observed from soil samples was attributed to the waste disposal. The total heterotrophic bacterial count was performed using l gram of soil from e-waste dumpsite in a 9-fold serial dilution using a spread plate method, in duplicates on dried nutrient agar plates and incubated at 30°C for 24hours. Centrimide agar plates were used to obtain Pseudomonas isolates and incubated at 37°C for 24 hours while MacConkey agar plates were used to isolate coliform bacteria, incubated at 0c for 48 hours. The physicochemical parameters were determined using standard methods.
Results: Seven (7) bacterial genera were isolated from the topsoil of the e-waste dumpsite and they were Staphylococcus, Pseudomonas, Kluyvera, Bacillus, Micrococcus, Chromobacterium and Pectobacteria species. Staphylococcus spp. had the highest percentage composition of 42.3% and Kluyvera spp, the lowest percentage composition of l.9% of bacterial isolates found in the topsoil of the electronic waste dumpsite. The total heterotrophic bacterial count ranged from l.30 x l06 to l.97 x l06 cfu/g, total coliform count was 3.05 x l03 to 7.98 x l03 cfu/g and total Pseudomonas count ranged from l.00 x l02 to 2.88 x l03 cfu/g with a significant difference at .05 probability level to the control samples. The temperature ranged from 27.67±0.580C to 28.00±l.000C with a control of 29.00±l.000C, pH value ranged from 6.33±0.58 with a control of 7.00±0.00. The pH values were lower than the control indicating that the soil was slightly acidic to neutral. Moisture content had 4% with a control of 2.7%, an organic matter of l7.33±0.58 with a control of 4.47±0.58.
Conclusion: The presence of the isolated organisms could cause public health risk or environmental hazard. Proper education and legislations on handling of e-waste in the society should be intensified to forestall waste related problems along the food chain.
Akintokun AK, Onatunde OO, Shittu OB, Okeyode IC,Taiwo MO. Bioaccumulation of Heavy Metals using Selected Organisms Isolated from Electronic Waste Dumpsite of two South Western States in Nigeria. Applied Environmental Research. 2017; 39(2):29-40.
Abebe NK, Endalkachew. Impact of Biomass Burning on Selected Physicochemical Properties of Nitisol in Jimma Zone, Southwestern Ethiopia. Intl Research J Agric Sc and Soil Sc. 2011; 1(9):394-401.
Ugboma CJ, Ndubuisi-Nnaji UU, Ezenobi NO. Microbial burden of air and soil in the vicinity of some waste receptacles in Uyo, Nigeria. Intl J Agric and Earth Sc. 2018; 4(3):2489.
Williams JO, Hakam K. Microorganisms associated with dumpsites in Port Harcourt Metropolis, Nigeria. Ecolog. Nat Env. 2016; 8(2):9-12.
Cappuccino JG, Sherman N. Microbiology: A Laboratory Manual. Pearson Education Incorporated, USA. 2012;193.
Oyeyiola GP. Practical Soil Microbiology Manual. Department of Microbiology, University of Ilorin, Ilorin, Nigeria. 2004;1-5.
AOAC. Official Method of Analysis: Association of Analytical Chemists, l9th Ed. Washington DC; 2012.
Riegel GM, Svejcar TJ, Busse MD. Does the presence of Wyethia mollis affect growth of Pinus Jeffreyi seedlings? Western North American Naturalist. 2002; 62(2):141-150.
Akpoveta OV, Osakwe SA, Okoh BE, Otuya BO. Physicochemical characteristics and levels of some heavy metals in soils around metal scrap dumps in some parts of Delta State., Nigeria. J Appl Sc and Environ Mgt. 2009;14(4):57-60.
Taiwo MO, Onatunde OO, Bamisile O, Nwachukw BC, Sakariyau AO. Assessment of soil microorganisms, heavy metal levels and natural radionuclei concentrations of three electronic waste dumpsites in Nigeria. Intl. J. Microbial. Research. 2018;9(3):81-88.
Ogbonna DN, Kii BC, Youdeowei, PO. Some physicochemical and heavy metal levels in soils of waste dump sites in Port Harcourt municipality and environs. J Appl Sc and Environ Mgt. 2009;l3(14):65-70.
World Health Organization (WHO). Air Monitoring Programme Designed for Urban and Industrial Area Published for Global Environmental Monitoring Systems by UNEP, WHO AND WMO; 1971.
Federal Environmental Protection Agency (FEPA). Guidelines and Standards for Environmental pollution control in Nigeria. Federal Republic of Nigeria. 1991;61-63.
Kabiru SR, Yakubu A, Lukman T, Akintola, Alegbemi M. Heavy metal contentin soil in Garki Area Council of Federal Capital Territory, Abuja Nigeria. Biochem. Anal. Biochem. 2015;4:197.
De Miguel EI, Irribaren E, Chacon A, Ordonez, Charlesworth S. Risk based evaluationof the exposure of children to trace elements in playgrounds in Madrid (Spain).Chem. 2007;66:505-513.
Ogboma DN, Igbemijie M, Isiminah N. Microbiological and physicochemical characteristics of the soils of waste collection sites in Port Hart city, Nigeria. Nig J Soil Sc. 2006;16:162-167.