Performance Evaluation of Non-Saccharomyces in Wine Production

Main Article Content

David N. Ogbonna
Janet O. Williams
Awengi Alabere

Abstract

Wines with different tastes, aromas, colours and acceptability produced through the fermentative action of yeasts are consumed worldwide Several studies have centred on the use of Saccharomyces sp. as the choice yeast for fermentation due to its desirable characteristics; however, in recent years, researches are being focused on alternatives to Saccharomyces sp. This study however was aimed at carrying out a performance evaluation of wine produced by the fermentative action of non-Saccharomyces (Meyerozyma guilliermondii and Pichia guilliermondii). The wines were produced using a mixture of pineapple juice and blended banana pulp as substrate.The fermentation process comprised of two set ups: one was fermented by Meyerozyma guilliermondii strain 1621 and the other was fermented by Pichia guilliermondii strain PAX-PAT 18S. An organoleptic (sensory) evaluation of both wines produced was carried out to ascertain the quality of the wines in comparison to a commercial wine. This was done using a 9-point hedonic scale rating. The wines were evaluated for their organoleptic characteristics including aroma, colour, clarity, taste and overall acceptability. The wine fermented by Meyerozyma guilliermondii had mean values of 7.3, 7.0, 6.4, 3.8 and 6.1 for aroma, colour, clarity, taste and overall acceptability, respectively. The wine fermented by Pichia guilliermondii had mean values of 6.2, 7.1, 5.5, 4.7 and 5.9 for aroma, colour, clarity, taste and overall acceptability, respectively. The commercial wine had mean values of 4.7, 6.6, 7.4, 7.4 and 6.5 for aroma, colour, clarity, taste and overall acceptability, respectively. Statistically, there was significant difference between the aroma of the wines (P≤0.05) while there were no significant differences in colour, clarity, taste and overall acceptability of the wines.  This study shows that acceptable wines can be produced using non-Saccharomyces yeasts like Meyerozyma guilliermondii and Pichia guilliermondii.

Keywords:
Wine production, pineapple and banana substrates, fermentation, non-Saccharomyces species (Meyerozyma guilliermondii and Pichia guilliermondii).

Article Details

How to Cite
Ogbonna, D. N., Williams, J. O., & Alabere, A. (2020). Performance Evaluation of Non-Saccharomyces in Wine Production. South Asian Journal of Research in Microbiology, 7(3), 47-54. https://doi.org/10.9734/sajrm/2020/v7i330175
Section
Original Research Article

References

Harding G. Wine Miscellany. Clarkson Potter Publishing, New York. 2005;5-9.

Noll RG. The wines of West Africa: History, technology and tasting notes. Journal of Wine Economics. 2008;3:85-94.

Obaedo ME, Ikenebomeh MJ. Microbiology and production of banana (Musa sapientum) wine. Nigerian Journal of Microbiology. 2009;23:1886-1891.

Chilaka CA, Uchechkwu N, Obidiegwu JE, Akpor OB. Evaluation and efficacy of yeast isolates from palm wine in diverse fruit wine production. African Journal of Food Science. 2010;4(12):764-774.

Martini A, Ciani M, Scorzetti G. Direct enumeration and isolation of wine yeasts from grape surfaces. American Journal of Enology and Viticulture. 1996;47(4):435-440.

Barata A, Seborro F, Belloch C, Malfeito- Ferreira M, Loureiro V. Ascomycetous yeast species recovered from grapes damaged by honeydew and sour rot. Journal of Applied Microbiology. 2008; 104(4):1182-1191.

Jolly NP, Augustyn OPH, Pretorius IS. The occurrence of non- Saccharomyces cerevisiae yeast species over three vintages in four vineyards and grape musts from four production regions of the Western Cape, South Africa. South African Journal of Enology and Viticulture. 2017; 24(2):8.

Benito S. The impact of Torulaspora delbrueckii yeast in wine making. Journal of Applied Microbiology and Biotechnology. 2018;102:3081-3094.

Ciani M, Comitini F. Non- Saccharomyces wine yeasts have a promising role in biotechnological approaches to wine making. Journal of Analytical Microbiology. 2011;61:25-32.

Comitini F, Gobbi M, Domizio P, Romania C, Lencioni L, Mannazzu I, Ciani M. Selected non- Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae. Journal of Food Microbiology. 2011;28:873-882.

Reza F, Zaman W, Abdullah WNW, Yang TA. Physical and sensory analysis of high fiber bread incorporated with Jackfruit rind flour. Journal of Food Science and Technology. 2013;1(2):30-36.

Jolly NP, Valera C, Pretorius IS. Not your ordinary yeast: non- Saccharomyces yeasts in wine production uncovered. Journal of the Federation of European Microbiological Societies Yeast Research. 2014;14:215-237.

Garcia M, Esteve-Zarzoso B, Arroyo T. Non- Saccharomyces yeasts: Biotechnological role for wine production. in grape and wine biotechnology; Intech Open: London, United Kingdom. 2016;11: 1-25.

Valera C. The impact of non- Saccharomyces yeasts in the production of alcoholic beverages. Journal of Applied Microbiology and Biotechnology. 2016; 100:9861-9874.

Padilla B, Gil JV, Manzanares P. Past and future of non- Saccharomyces yeasts from spoilage microorganisms to biotechnological tools for improving wine aroma complexity. Journal of Frontiers in Microbiology. 2016;7:411-431.

Padilla B, Zulian L, Ferreres A, Pastor R, Esteve-Zarzoso B, Beltran G, Mas A. Sequential inoculation of native non- Saccharomyces and Saccharomyces cerevisiae strains for wine making. Journal of Frontiers in Microbiology. 2017;8:1293-1305.

Petruzzi L, Capozzi V, Berbegal C, Corbo MR, Bevilacqua A, Spano G, Sinigaglia M. Microbial resources and enological significance: Opportunities and benefits. Journal of Frontiersin Microbiology. 2017; 8:995-1008.

Ciani M, Comitini F. Use of Non- Saccharomyces Yeasts in Red Wine making. In Red Wine Technology; Academic Press: Cambridge, M A, USA. 2019;4:51-68.

Roudil L, Russo P, Berbegal C, Albertin W, Spano G, Capozzi V. Non-Saccharomyces Commercial Starter Cultures: Scientific Trends, Recent Patents and Innovation in the Wine Sector. Journal of Recent Patents on Food nutrition and Agriculture. 2019;10:1-13.

Alabere A, Ogbonna DN, Williams JO. Screening of yeast cells for the production of wine from banana and pineapple substrates. Journal of Advances in Microbiology. 2020;20(7):38-55.

Benito S, Morata A, Palomero F, Gonzales MC, Suarez-Lepe JA. Formation of vinylphenolic pyranoanthocyanins by Saccharomyces cervisiae and Pichia guilliermondii in red wines produced following different strategies. Journal of Food Chemistry. 2011;124:15-23.

Benito S, Hofmann T, Laier M, Lochbuhler B, Schuttler A, Ebert K, Fritsch S, Rocker J, Rauhut D. Effect and quality and composition of Riesling wines fermented by sequential inoculation with non- Saccharomyces and Saccharomyces cerevisiae. Journal of European Food Research and Technology. 2015;241:707-717.

Lim J. Hedonic scaling: A review of methods and theory. Journal of Food Quality Preference. 2011;22:733-747.

Cardello AV, Schutz HG. Numerical scale- point location for constructing the LAM (labeled affective magnitude) scale. Journal of Sensory Studies. 2004;19:341-346.

Lim J, Wood A, Green BG. Derivation and evaluation of a labelled hedonic scale. Journal of Chemical Sensory. 2009;34: 739-751.

Lim J, Fujimaru T. Evaluation of the labeled hedonic scale under different experimental conditions. Journal of Food Preference. 2010;21:521-530.

Swiegers JH, Bartowsky EJ, Henschke PA, Pretorius IS. Yeast and bacterial modulation of wine aroma and flavour. Australian Journal of Grape Wine Research. 2005;11(2):139-173.

Cabrita MJ, Costa Freitas AM, Laureano O, Borsa D, Di Stefano R. Aroma compounds in varietal wines from Alentejo, Portugal”. Journal of Food Composition and Analysis. 2007;2(5):357-390.

Tao YS, Liu YQ, Li H. Sensory characteristics of Cabernet Sauvignon dry red wine from Changli Country (China). Journal of Food Chemisty. 2009;114:565-569.

Miele A, Rizzon LA. Discrimination of Brazillian red varietals wine according to their sensory descriptors. Journal of Cienc Agrotec. 2011;35:1172-1177.

Van Leeuwen C, Friant P, Chone X, Tregoat O, Koundouras S, Dubourdieu D. Influence of climate, soil and cultivar on terroir. American Journal of Enological Viticulture. 2004;55:207-217.

Lawless HT. Descriptive analysis of complex odours: Reality, model or illusion? Journal of Food quality Preference. 1999;10:325-332.

Aminu Z, Yabaya A, Mohammed SSD, Bobai M. Quality assessment of water melon (Citrulus lanatus) wine produced using Saccharomyces cerevisiae isolated from palm wine. Journal of Biomaterials. 2019;2(2):65-73.

Dellacassa E, Trenchs O, Farina, L, Debernardis F, Perez G, Boido E, Carrau F. Pineapple (Ananas comosus I. Merr.) wine production in Angola: Characterisation of volatile aroma compounds and yeast native flora. International Journal of Food Microbiology. 2017;241:161-167.

Morata A, Escott C, Banuelos MA, Loira I, del Fresno JM, Gonzalez C, Suarez-Lepe JA. Contribution of non-Saccharomyces yeasts to wine freshness: A review. Journal of Biomolecules. 2019;10(34):1-5.

Garcia A, Carcel C, Dulau L, Samson A, Aguera E, Agosin E, Gunata Z. Influence of a mixed culture with Debaromyces vanriji and Saccharomyces cerevisiae on the volatiles of a Muscat wine. Journal of Food Science. 2002;67:1138-1143.

Bisson LF, Walker GA. Advances in Fermented Foods and Beverages. Improving quality, technologies and health benefits. Woodhead Publishing Series in Food Science, Technology and Nutrition. 2015;435-476.

Dharmadhikari M. Wine aging. Midwest grape and wine industry.

Available:https://www.extension.iastate.edu/wine/wine-aging

Assessed 13 July, 2020.