Chemical Composition and Efficacy of Ocimum basilicum L. Essential Oil against Stored Rice (Oryza sativa L.) Molds Sold in Ngaoundere (Cameroon)

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Nadege Donkeng Nganou
Steve Francky Sohanang Nodem
Thierry Marcel Ntsamo Beumo
Alphonse Tegang Sokamte
Leopold Ngoune Tatsadjieu


The aimed of this study to evaluate the antifungal activity of essential oils of Ocimum basilicum L. on some fungi isolated from commercial rice sold in Cameroon. Fungal isolates were primarily identified based on morphological characteristics, while representative isolates were identified using PCR-based methods. Essential oils were extracted by hydro-distillation, chemical composition was analyzed by gas chromatography coupled with mass spectrometry (GC-MS) and antifungal activity against isolated fungi were assessed using the micro-atmospheric method. The percentage of frequency and abundance varied with the sample analyzed and the fungi detected. A total of 85 fungal isolates of eight genera including Aspergillus, Fusarium, Penicillium, Rhizopus, Cladosporum, Mucor Alternaria and Acremonium in decreasing order of predominance were identified. The most fungal species identified were Aspergillus flavus, A. fumgatis, A. niger, A. carbonarius, Fusarium oxysporum, F. graminarum, F. moniliforme, Penicillium citrinum, P. expansum, Rhizopus oryzea, Cladosporum sp, Mucor hiemalis, Alternaria solani, Acremonium murorum. More than 61% (A. flavus, A. niger, P. citrinum) and 44% (M. hiemalis, A. flavus, A. niger) of analyzed samples contain respectively aflatoxin B1, AFB1 (0 - 17.3 µg/kg) and ochratoxin A, OTA (0 - 5.2 µg/kg). Citrinin was not detected. The main compounds identified in O. basilicum EO are eugenol (30.6), linalool (29.5), cineole (14.4), Terpinen-4-ol (5.6), 2-norpinen (4.1), Cadinen (3.3), Limonen (2.4) and (E)-β-Ocimene (2.2). The O. basilicum EO exhibit antifungal activity with the MIC range from 750-2000 ppm. This activity varies with the strains and the concentration of EO. The increased prevalence of mycotoxigenic fungi in rice, a highly consumed food grain in Africa, poses serious health concerns to the general public. The O. basilicum EO can therefore be used as alternatives to synthetic pesticide in rice storage.

Ocimum basilicum, essential oil, rice, molds, mycotoxins, Ngaoundere, Cameroon.

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Nganou, N. D., Nodem, S. F. S., Beumo, T. M. N., Sokamte, A. T., & Tatsadjieu, L. N. (2020). Chemical Composition and Efficacy of Ocimum basilicum L. Essential Oil against Stored Rice (Oryza sativa L.) Molds Sold in Ngaoundere (Cameroon). South Asian Journal of Research in Microbiology, 7(3), 55-65.
Original Research Article


Makun HA, Dutton MF, Njobeh PB, Phoku JZ, Yah CS. Incidence, phylogeny and mycotoxigenic potentials of fungi isolated from rice in niger state, nigeria. Journal of Food Safety. 2011;31:334–349.

Piyo A, Udomsilp J, Khang-Khun P, Thobunluepop P. Antifungal activity of essential oils from basil (Ocimum basilicum Linn.) and sweet fennel (Ocimum gratissimum Linn.): Alternative strategies to control pathogenic fungi in organic rice. Asian Journal of Food and Agro-Industry. 2009;2:S2–S9.

Surekha M, Saini K, Reddy VK, Reddy AR, Reddy SM. Fungal succession in stored rice (Oryza sativa Linn.) fodder and mycotoxin production. African Journal of Biotechnology. 2011;10:550–555.

Taligoola HK, Ismail MA, Chebon SK. Mycobiota and aflatoxins associated with imported rice grains stored in Uganda. Czech Mycology. 2011;62:93–107, DOI:10.33585/CMY.63109.

Makun HA, Gbodi TA, Akanya OH, Salako EA, Ogbadu GH. Fungi and some mycotoxins contaminating rice (Oryza Sativa) in Niger State, Nigeria. African Journal of Biotechnology. 2007;6:099–108. DOI:10.4314/ajb.v6i2.56106.

Getachew A, Chala A, Hofgaard IS, Brurberg MB, Sulyok M, Tronsmo AM. Multimycotoxin and fungal analysis of maize grains from south and southwestern Ethiopia. Food Addit Contam Part B Surveill. 2017;11:64–74. DOI:10.1080/19393210.2017.1408698.

Nguefack J, Nguikwie SK, Fotio D, Dongmo B, Zollo PHA, Leth V, Nkengfack AE, Poll L. Fungicidal potential of essential oils and fractions from Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris to control Alternaria padwickii and Bipolaris oryzae, two seed-borne fungi of rice (Oryza sativa L.). Journal of Essential oil Research : JEOR. 2007;19:581–587, DOI:10.1080/10412905.2007.9699336.

Reddy KRN, Reddy CS, Abbas HK, Abel CA, Muralidharan K. Mycotoxigenic fungi, mycotoxins, and management of rice grains. Toxin Reviews 2008;27:287–317. DOI:10.1080/15569540802432308.

Dambolena JS, Zunino MP, López AG, Rubinstein HR, Zygadlo JA, Mwangi JW, Thoithi GN, Kibwage IO, Mwalukumbi JM, Kariuki ST. Essential oils composition of Ocimum basilicum L. and Ocimum gratissimum L. from Kenya and their inhibitory effects on growth and fumonisin production by Fusarium verticillioides. Innovative Food Science & Emerging Technologies. 2010;11:410–414. DOI:10.1016/j.ifset.2009.08.005.

Neveen HA, El-Kassem LA, Khalil M. Chemical composition and antifungal activity of Syzygium aromaticum L. essential oil. Iranian Journal of Medicinal and Aromatic Plants. 2017;33:552–561.

Sajjadi SE. Analysis of the essential oils of two cultivated basil (Ocimum basilicum L.) from Iran. Daru. 2006;14:128–130.

Ahmad K, Khalil A. talha; Yusra; Somayya, R. Antifungal, phytotoxic and hemagglutination activity of methanolic extracts of Ocimum basilicum. Journal of Traditional Chinese Medicine. 2016;36:794–798. DOI:10.1016/S0254-6272(17)30017-1.

AOAC AOAC: Official Methods of Analysis; 15th Edition.; Kenneth Helrich: Virginia-USA. 1990;1.

Pitt JI, Hocking AD. Fungi and Food Spoilage; Springer US: Boston, MA. ISBN: 2009;978-0-387-92206-5.

Raper KB, Fennell DI. The genus Aspergillus.; Williams and Wilkins, company: Baltimore, Maryland; 1965.

Iqbal SZ, Asi MR, Hanif U, Zuber M, Jinap S. The presence of aflatoxins and ochratoxin A in rice and rice products; and evaluation of dietary intake. Food Chemistry. 2016;210:135–140. DOI:10.1016/j.foodchem.2016.04.104.

Reiter EV, Vouk F, Böhm J, Razzazi-Fazeli E. Aflatoxins in rice – A limited survey of products marketed in Austria. Food Control. 2010;21:988–991. DOI:10.1016/j.foodcont.2009.12.014

Kéita SM, Vincent C, Schmit JP, Bélanger A. Essential oil composition of Ocimum basilicum L., O. gratissimum L. and O. suave L. in the Republic of Guinea. Flavour and Fragrance Journal. 2000;15:339–341, DOI:10.1002/1099-1026(200009/10)15:5<339::AID-FFJ922>3.0.CO;2-H.

Akgül A. Volatile oil composition of sweet basil (Ocimum basilicum L.) cultivating in Turkey. Food / Nahrung. 1989;33:87–88. DOI:10.1002/food.19890330129.

Naghdi Badi H, Yazdani D, Ali SM, Nazari F. Effects of spacing and harvesting time on herbage yield and quality/quantity of oil in thyme, Thymus vulgaris L. Industrial Crops and Products. 2004;19:231–236. DOI: 10.1016/j.indcrop.2003.10.005