Evaluation of Microbial Loads and Physico-Chemicals of Cassava Mill Effluent Simulated Soil
South Asian Journal of Research in Microbiology,
Evaluation of microbial loads and physico-chemicals of cassava mill effluent simulated soil was carried out using standard microbiological and biochemical techniques. This was to determine the effect of cassava mill effluent (CME) on rhizosphere microbial loads, physicochemical properties, nitrogenous salt and heavy metals. The results showed that CME effect on the physicochemical determinants (pH, Ca, Mg, K) and heavy metal determinant (Fe, Zn, Co, Ni, Pb and Mn) was concentration dependents. The nitrogenous salts (NO3, NH4+ and NO2) levels progressively increased with no significant differences (p>0.05 ANOVA). The microbial isolates were: Saccharomyces sp, Mucorindicus, Fusarium sp and Gliocladium sp for the fungal group. The bacterial group were Chromobacterium sp, Corynebacterium sp, Bacillus sp, Acinetobacter sp and Escherichia coli while the nitrogen-fixing bacterial group were Azotobacter sp., Azospirillum sp., Frankia sp., Bradyrhizobium sp., Hebaspirillum sp., Cyanobacteria (or blue green algae), Anabaena sp, Nostoc sp., Clostridium sp. and Rhizobium sp. There was no significant differences (p>0.05) in the rhizosphere microbial load across the concentration gradient at the CME-simulated plot phyto-remediated by Centrosema pubesscens and Calopogonium mucunoides. Agricultural wastes such as cassava mill effluent should be properly treated before discharging to the environment in other to prevent the loss of nitrogen-fixing bacteria and total heterotrophic bacterial genera that could be of immense importance to man.
- nitrogen-fixing bacteria
- heavy metal
- nitrogenous salts and cassava mill effluent (CME).
How to Cite
Noorjahan CM, Jamuna S. Physico-chemical characterisation of brewery effluent and its degradation using native fungus-aspergillus niger, aquatic plant-water hyacinth-eichhorniasp and green mussel–pernaviridis. J. Enviro. Earth Sci. 2012; 2(4):31-40.
Izonfuo WA, Bariweni PA, George DMC. Soil contamination from cassava wastewater discharges in a rural community in the Niger Delta, Nigeria. J. Appl. Sci. Enviro. Management. 2013; 17(1):105-110.
Okunade DA, Adekalu KO. Characterization of cassava-waste effluents contaminated soils in Ile-Ife, Nigeria. European Intern. J. Sci, Tech. 2014;3(4):173-182.
Bhattacharya T, Chakraborty S, Ncha T. Physicochemical characterization of ground water of Anand district, Gujarat, India. Inter. J. Environ. Sci. 2012;1:28-33.
Pradeep MR, Narasimha G. Effect of leather industry effluents on soil microbial and protease activity. J. Environ. Biol. 2012;33:39-42.
Iram S, Ahmad I, Stuben D. Analysis of mines and contaminated agricultural soil samples for fungal diversity and tolerance to heavy metals. Pakistan J. Bot. 2009; 41: 885-895.
Adejumo BA, Ola FA. The effect of cassava effluent on the chemical composition of agricultural Soil. 2012;220-226
Chaturvedi RK, Sharma KP, Sharma K, Bhardwaj SM, Subhasini S. Plankton community of polluted waters around Sanganer, Jaipur. J. Enviro Poll. 1999; 6:77-84.
Sponza DT. Necessity of toxicity assessment in Turkish industrial discharges (examples from metal and textile industry effluents). J. Environ. Monitoring Assess. 2002;73(1):41-66.
Agbo BE, Ogar AV, Itah AY, Brooks AA, Akonjor MA. Assessment of the effects of cassava mill effluent on the soil and its microbiota in biase local government area of Cross River State, Nigeria. World J. Adv. Res. Reviews. 2019;1(2):034–044.
Osakwe SA. Effect of cassava processing mill effluent on physical and chemical properties of soils in Abraka and Environs, Delta State, Nigera. Res. J. Chem. Sci. 2012;2(11):7-13.
John RC, Itah AY, Essien JP, Ikpe DI. Fate of nitrogen-fixing bacteria in crude oil contaminated wetland ultisol. Bulletin Enviro. Contam. Toxicol. 2011;87:343-353.
Association of Official Analytical Chemists (AOAC). Official Method of Analysis 14th ed., Washington, D.C. Association of Official Analytical Chemists; 1984.
Sofowora A. Medicinal plant and traditional medicine in Africa, Ibadan, Nigeria. Spectrum Books Limited. 1993;289.
Egwaikhide PA, Gimba CE. Analysis of the phytochemical content and antimicrobial activity of Plectrabthusglandulosis whole plant. Middle-East J. Sci. Res. 2007;2(4): 135-138.
Mofunanya AJ, Abia-Bassey LN, Akpan JO. Microbial sensitivity to extracts of Mitracarpusscaber. Global J. Pure Appl. Sci. 2007;13(1):89-93.
Gafar MK, Hassan LG, Danoggo SM, Hod AU. Amino acid estimation and phytochemical screening of Indigofera astragoline leaves. J. Chem. Pharma. Res. 2010;2(5):277-285.
Chandrapp CP, Schiplasheree CB, Karthik MR, Govindappa M, Sadanmanda TS. Antibacterial and anti-oxidant activities of Adiantum pedatum. J. Phytol. 2011;3(1): 26-32.
Mofunanya AJ, Nta AI. Determination of phytochemicals in Telfaria occidentalis, Amaranthus hybridus, Phaseolus vulgaris and Sphenostylisstenocapa inoculated with Telfaria mosoaic virus (TeMV). Intern. J. Nat. Appl. Sci. 2011;6(2):1-8.
Agbo BE, Mboto CI. Phytochemical and antibacterial evaluation of selected locally produced herbal medicines sold in Calabar, Nigeria. Arch. Appl. Sci. Res. 2012;4(5):1974-1990.
Brooks AA, Iyakndue ML, Unimke AA, Agbo BE. Rubber effluent bio-analyses and its impacts on the microbial community structure of the soil in Calabar, Nigeria. Asian J. Environ Ecol. 2017;4(3):1-9.
Iyakndue ML, Brooks AA,Unimke AA, Agbo BE. Effects of palm oil mill effluent on soil microflora and fertility in Calabar–Nigeria. Asian J. Biol. 2017;2(3):1-11.
Qishlaqi A, Moore F. Statistical analysis of accumulation and sources of heavy metals occurrence in agricultural soils of Khoshk River Banks, Shiraz, Iran. American-Eurasian J. Agric. Environ Sci. 2007;2(5): 565-573.
Akpan JF, Eyong MO, Isong IA. The impact of long-term cassava mill effluent discharge on soil pH and microbial characteristics in Cross River State. Asian J. Soil Sci. Plant Nutr. 2017;2(1):1-9.
Obire O, Nwaubet O. Effect of refined petroleum hydrocarbon on soil physicochemical and bacteriological characteristics, J. Appl. Sci. Environ. Management. 2002;6(1):39-44.
Eze VC, Okpokwasili GC. Microbial and other related changes Niger Delta River sediment receiving industrial effluents, Continental J. Microbiol. 2010;4(10):15-24.
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