Incidence of Methicillin Resistant Staphylococcus aureus Isolated from Nostrils of Mouau Students
South Asian Journal of Research in Microbiology,
Aim: This study is carried out to determine the prevalence of nasal carriage of staphylococcus aureus among Michael Okpara University of Agriculture Umudike (MOUAU) students and determine the antibiotics susceptibility pattern.
Methods: Nasal swab specimens collected from the anterior nares were cultured on mannitol salt agar. S.aureus isolates were identified by mannitol fermentation, coagulase positivity and catalase positivity. Antimicrobial susceptibility test was performed on Meuller-Hinton Agar (MHA) by modified Kirby-Bauer disc diffusion method.
Result: Out of the 100 swabs collected, S.aureus was isolated from 60% of the samples. Among colonized students, 63.3% (n=38) were from male students whereas 36.7% (n=22) were from female. The antibiotic susceptibility pattern of the Staphylococcus aureus isolates indicates that Staphylococcus aureus isolates showed high rate of sensitivity towards antibiotics as follows; Gentamycin (81.67%), followed by Ciprofloxacin (80%), Levofloxacin (76.67%), Ceftriaxone (58.33%), and resistance towards antibiotics Imipenem (100%) followed by Cefotaxime (90%), Cefexime (83.33%), Cefoxitin (65%), azithromycin (55%) and cefuroxime (50%).
Conclusion: This study showed a high rate of nasal carriage of Staphylococcus aureus among MOUAU students and a high incidence of antibiotics resistant Staphylococcus aureus carriage.
- staphylococcus aureus
- methicillin resistant
- nasal swab
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Emaneini M, Hosseinkhani F, Jabalameli F, Nasiri MJ, Dadashi M, Pouriran R. Prevalence of vancomycin-resistant enterococcus in Iran: A systematic review and meta-analysis. European Journal of Clinical Microbiology and Infectious Diseases. 2016;35:1387-92.
Espinosa-Gongora C, Dahl J, Elvstrom A, Van-Wamel WJ, Guardabassi L. Individual predisposition to Staphylococcus aureus colonization in pigs on the basis of quantification, carriage dynamics, and serological profiles. Applied Environmental Microbiology 201581:1251-6.
Agumas S, Tamrat A, Adane M. Nasal carriage rate of methicillin resistant Staphylococcus aureus among Dessie Referral Hospital Health Care Workers; Dessie, Northeast Ethiopia Antimicrobial Resistance and Infection Control. 2013;2:25-29.
Calfee DP, Salgado CD, Milstone AM, Harris AD, Kuhar DT, Moody J. Strategies to prevent methicillin-resistant Staphylococcus aureus transmission and infection in acute care hospitals. Update on Infectious Control Hospital Epidemiology. 2014;35:772-96.
Annati E, Arzanlou M, Habibzadeh S, Mohammadi S, Ahadi P, Mohammadi-Ghalehbin B. Nasal colonization of mecApositive, oxacillin- susceptible, methicillin-resistant Staphylococcus aureus isolates among nursing staff in an Iranian teaching hospital. American Journal of Infection Control. 201341:1122-4.
Castro A, Komora N, Ferreira V, Lira A, Mota M, Silva J. Prevalence of staphylococcus aureus from nares and hands on health care professionals in a Portuguese Hospital. Journal of Applied Microbiology. 2016;121(3):831-9.
Ahanjan M, Abdollahi S, Abdolian H. Prevalence of methicilin resistant staphylococcus aureus isolated from hand and nose of hospital health worker. Journal of Gorgan University of Medical Science. 2014;16:131-136.
De-Boeck H, Vandendriessche S, Hallin M, Batoko B, Alworonga JP, Mapendo B. Staphylococcus aureus nasal carriage among healthcare workers in Kisangani, the Democratic Republic of the Congo. European Journal of Clinical Microbiology and Infectious Diseaases. 2015;34:1567-1572.
Lari AR, Pourmand MR, Moghadam SO, Abdossamadi Z, Namvar AE, Asghari B. Prevalence of PVL-containing MRSA isolates among hospital staff nasal carriers. Laboratory Medical. 2011;42:283-286.
The future of natural products as a source of new antibiotics. Andriy Luzhetskyy, Stefan Pelzer, Andreas Bechthold Current opinion in investigational drugs (London, England: 2000). 2007;8(8): 608-613.
Bergstrom R. The role of the pharmaceutical industry in meeting the public health threat of antibacterial resistance. Drug Resistance Update. 2011;14:77-78.
Brown DF, Edwards DI, Hawkey PM et al. Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA). J Antimicrob Chemother. 2005;56(6):1000-18. [ Links ]
Rai PK, Sharma AP, Tripathi BD. Comparative assessment of Azolla pinnata and Vallisneria spiralis in Hg removal from G.B. Pant Sagar of Singrauli Industrial region, India; 2007.
Ugwu MC, Anie CO, Ibezim EC, Esimone CO. Antimicrobial evaluation of methicillin-resistant staphylococcus aureus nasal carriage amongst healthy students in agbor, delta state, Nigeria. Archives of Clinical Microbiology. 2016: 7:1989-8436.
Nsofor CA, Ezeh IO, Nwabia BC. Nasal carriage of multidrug- resistant staphylococcus aureus among apparently healthy school children in elele, Nigeria. Advances in Medical and Biological Science Research, 2013;3(1): 29-35.
Ononuga A, Temedie TC. Nasal carriage of multi-drug resistant Staphylococcus aureus in healthy inhabitants of Amasoma in Niger delta region of Nigeria. African Health Science. 2011;11(2):176-181.
Chijioke NA, Vivian NN, Calistus N. Nasal carriage of staphylococcus aureus among apparently healthy school children in owerri metropolis, Nigeria. Cell Science and Report. 2015;2(5):2-7.
B batabyal, Shilpa Biswas, B Mandal. Isolation of imipenem resistant Staphylococcus aureus from post-operative pus sample in oral and maxillofacial infections (October 2012) Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2012;3(4):896-900.
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