A Malian Rice Seed Endophyte Bacteria Control Efficiently the Growth of Xanthomonas oryzae pv oryzae and Xanthomonas oryzae pv oryzicola
Djeneba Ouattara
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Sognan Dao
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Amadou Hamadoun Dicko
Faculté d’Agronomie et de Médecine animale, Université de Ségou, Ségou, Mali.
Adounigna Kassogué
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Djeneba Nantoumé
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Ibrahima Mallé
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Mamadou W. Bagayogo
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Seydou Koné
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Bakaye Doumbia
Centre Régionale de Recherche Agronomique (CRRA) de Niono, Institut d’économie Rurale, Niono (Ségou), Mali.
Fatouma Alhadji Faradji
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Kangaye Amadou Diallo
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Ousmane Diarra
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Salimatou Samaké
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Rokiatou Fané
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Moctar Coulibalily
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Christiane Dembelé
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
Amadou Hamadoun Babana *
Laboratoire de Recherché en Microbiologie et Biotechnologie Microbienne, Faculté des Sciences et des Techniques, BPE3206, Mali.
*Author to whom correspondence should be addressed.
Abstract
Aims: In Mali, rice (Oryza sativa L.) is a very important cereal in the food system and important losses in rice production are caused by bacterial diseases. Despite the important rice losses in production and the proven ability of some bacteria to control rice pathogens, in Mali very few studies have been carried out in biocontrol. With the aim of improving food security, the objective of this work is to select, at least one endophyte bacteria with great capacity to inhibit the growth Xanthomonas oryzae pv oryzae and Xanthomonas oryzae pv oryzicola.
Study Design: This experimental research involving a Malian local rice cultivar named Kogoni and biocontrol endophyte bacteria isolated from rice in Mali.
Pace and Duration of Study: This research was carried out in greenhouse at LaboREM-Biotech and in the field at the Office of irrigated perimeters of Baguineda (OPIB) for 3 years.
To achieve this objective, pathogenic bacteria (Xoo and Xoc) circulating in rice fields of Mali were identified, endophyte bacteria from the bacterial collection of the LaboREM with high antagonistic activities against the identified pathogens were selected, and the efficacy of the selected endophytes to inhibit the growth of these pathogens in vitro were tested.
Results: A total of five isolates, including 3 from Xoo and 2 from Xoc, were isolated in Niono from two varieties of rice: Kogoni91-1 and Adny11. No isolate characteristic of Xanthomonas oryzae was observed on samples from Baguineda. The three selected rice endophytes were tested in vitro to assess their effectiveness in controlling Xoo and Xoc. Following this test, 100% of the endophytes showed significant antimicrobial activity against Xoo and Xoc with an inhibition diameter varying between 6 and 28.5mm. endo Ad9 was selected for its strong ability to inhibit the growth of both pathogens at the same time.
Keywords: Rice, Xanthomonas oryzae pv oryzae, Xanthomonas oryzae pv orizycola, endophytes, growth inhibition