Identification and Characterization of Water Borne Pathogen
Shivangi Saxena
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan-304022, India.
Dipjyoti Chakraborty *
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan-304022, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: To carry out isolation and characterization of bacteria from water samples.
Study Design: Collection of water samples from different water sources, isolation of bacteria and establishment of pure culture, identification of bacteria
Place and Duration of Study: Sample collected from Hingotiya and Palai village of Tonk district, Rajasthan July-August, 2018.
Methodology: For initial screening of bacterial population, standard methods namely serial dilution and streaking methods were used, for identification, colony morphology and molecular characterization (16s rRNA gene) was done.
Results: The bacteriological analysis revealed that Enterobacter cloacae was present in the collected tap-water.
Conclusion: Presence of E. cloacae in tap water necessitates recommendation of frequent sanitation, treatment, management of water sources, and routine regular bacteriological assessment of all water sources for consumption as a drinking water.
Keywords: Enterobacter cloacae, 16s rRNA gene, potable water, water borne pathogen, water borne diseases