Screening of Antimicrobial Activity of Murraya koenigii Leaf Extracts Against Pathogenic Bacterial Strains Staphylococcus aureus and Escherichia coli Isolated from Contaminated Water
Vini Mary Varghese
Department of Microbiology, Agro Bio Tech Research Centre Ltd (ABTEC), Poovanthuruthu, Kottayam, Kerala 686012, India.
Hemand Aravind *
Integrated Bio Chemical Laboratory, Agro Bio Tech Research Centre Ltd (ABTEC), Poovanthuruthu, Kottayam, Kerala -686012, India.
Saritha SS
Department of Microbiology, Agro Bio Tech Research Centre Ltd (ABTEC), Poovanthuruthu, Kottayam, Kerala 686012, India.
Mithilesh Jaiswal
Department of Microbiology, Agro Bio Tech Research Centre Ltd (ABTEC), Poovanthuruthu, Kottayam, Kerala 686012, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: Murraya koenigii is a widely used plant both as a potential medicinal agent and also for common cooking purposes. Aim of this present study was to determine the antimicrobial activity of Murraya koenigii leaf extracts on Staphylococcus aureus and Escherichia coli.
Study Design: Screening and isolation of pathogenic bacterial strains from contaminated water. Preparation of Murraya koenigii leaf extracts using petroleum ether, acetone and ethyl acetate by using serial extraction method with Soxhlet apparatus.
Place and Duration of Study: Department of microbiology, Agro biotec research centre Ltd, Poovanthuruthu, Kottayam, Kerala, India, between 2014 January to 2014 May.
Methodology: Staphylococcus aureus and Escherichia coli were the bacterial strains used in this study. Morphological and biochemical analysis of microorganisms were conducted to identify the strains. Leaf extracts (petroleum ether, acetone and ethyl acetate) of Murraya koenigii were screened using MHA disc diffusion methods.
Results: Various concentration of plant extracts were used to check its activity against isolated pathogens. Acetone extract of curry leaves exhibit maximum zone of inhibition against Staphylococcus aureus and petroleum ether extracts shown maximum inhibition against Escherichia coli.
Keywords: Murraya koenigii, antimicrobial activity, Staphylococcus aureus, Escherichia coli