First Case of Qnr B6 and Qnr B7 Genes in Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Abidjan, Côte D'ivoire
Gadou Victoire *
Department of Biosciences, Laboratory of Biochemical Pharmacodynamics, Felix Houphouët-Boigny University, Abidjan, 22 BP 582 Abidjan 22, Côte d’Ivoire and Pasteur Institute of Côte d’Ivoire, 08 BP 1563 Abidjan 08, Côte d'Ivoire.
Toty Abalé Anatole
Pasteur Institute of Côte d’Ivoire, 08 BP 1563 Abidjan 08, Côte d'Ivoire.
Konan Fernique
Tiékoura Konan Bertin
Pasteur Institute of Côte d’Ivoire, 08 BP 1563 Abidjan 08, Côte d'Ivoire.
Ouattara Mohamed Baguy
Pasteur Institute of Côte d’Ivoire, 08 BP 1563 Abidjan 08, Côte d'Ivoire.
Guessennd Kouadio Nathalie
Pasteur Institute of Côte d’Ivoire, 08 BP 1563 Abidjan 08, Côte d'Ivoire and Department of Medical Science, Felix Houphouët-Boigny University, Abidjan, Côte d'Ivoire.
Djaman Allico Joseph
Department of Biosciences, Laboratory of Biochemical Pharmacodynamics, Felix Houphouët-Boigny University, Abidjan, 22 BP 582 Abidjan 22, Côte d’Ivoire and Pasteur Institute of Côte d’Ivoire, 08 BP 1563 Abidjan 08, Côte d'Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Aims: The aim of this study was to characterize fluoroquinolone resistance genes in enterobacteriaceae that produce extended-spectrum β-lactamases, isolated in Abidjan.
Place and Duration of Study: Pasteur Institute of Côte d'Ivoire and research unit on emerging tropical infectious diseases of Aix-Marseille University from January 2017 at July 2017.
Methodology: The study included 90 enterobacteriaceae producing extended-spectrum β-lactamases isolated from biological products from various hospital services in Abidjan. These strains have been pre-identified and stored at the Center for Biological Resources (CeReB) of Pasteur Institute of Côte d'Ivoire. The identification of the strains was confirmed using the mass spectrometry MALDI-TOF (MS) and the antibiotic sensitivity test was performed using Müeller Hinton's agar diffusion method. The fluoroquinolone resistant genes were detected by conventional PCR and then, sequenced.
Results: The strains studied were Escherichia coli (44), Klebsiella pneumoniae (31) and Enterobacter cloacae (15). High resistance rates to ceftriaxone (96.7%), cefotaxime (95.6%), aztreonam (95.6%) and cefoxitin (72.2%) were observed in all strains producing broad spectrum β-lactamases. The resistance rate to fluororquinolones represented by ciprofloxacin was 86.7%. The fluoroquinolone resistance genes detected were qnr A (3.3%) and qnr B (42.2%). Sequencing identified the qnr A1 (3.3%), qnr B1 (31.1%), qnr B6 (2.2%) and qnr B7 (1.1%) genes.
Conclusion: This study made it possible to identify fluoroquinolone resistance genes in enterobacteriaceae producing β-lactamases which have an extended spectrum in Abidjan.
Keywords: Enterobacteriaceae, fluoroquinolones, qnr B6, qnr B7, Abidjan