Isolation and Molecular Identification of Biodeteriogens Isolated from Painted Classroom Wall Surfaces in University of Port Harcourt, Nigeria
H. O. Stanley *
Department of Microbiology, University of Port Harcourt, Port Harcourt, Rivers State, Nigeria.
B. C. Anele
Department of Microbiology, University of Port Harcourt, Port Harcourt, Rivers State, Nigeria.
P. O. Okerentugba
Department of Microbiology, University of Port Harcourt, Port Harcourt, Rivers State, Nigeria.
O. M. Immanuel
Department of Biological Sciences, University of Africa, Toru-Orua, Bayelsa State, Nigeria.
C. J. Ugboma
Department of Microbiology, Rivers State University, Nkpolu, Port Harcourt, Rivers State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Painted wall surfaces provide breeding ground for diverse microbial flora capable of causing deterioration of the building and progressively impact on the health of inhabitant. This study was undertaken to determine the bacterial and fungal biodeteriogens of painted classroom wall surfaces in University of Port Harcourt, Nigeria. Bacterial and fungal biodeteriogens were isolated from classroom wall surfaces showing visible signs of deterioration and dampness. Internal Transcribed Spacer (ITS) region of the rDNA was used in the identification of the isolates. The bacterial deteriogens were identified as Myroides odoratus, Bacillus subtilis, Bacillus sp. and Alcaligenes aquatilis, while fungal deteriogens were identified as Aspergillus nomius and Trametes polyzona. Presence of microorganisms in classroom wall surfaces can impact on air quality of the learning environment.
Keywords: Biodeteriogens, ITS region of rDNA, deterioration, painted classroom.