https://journalsajrm.com/index.php/SAJRM <p style="text-align: justify;"><strong>South Asian Journal of Research in Microbiology</strong>&nbsp;<strong>(ISSN: 2582-1989)&nbsp;</strong>aims to publish high quality papers (<a href="/index.php/SAJRM/general-guideline-for-authors">Click here for Types of paper</a>) in all aspects of Microbiology. By not excluding papers based on novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled, OPEN peer-reviewed, open-access INTERNATIONAL journal.</p> South Asian Journal of Research in Microbiology en-US South Asian Journal of Research in Microbiology 2582-1989 https://journalsajrm.com/index.php/SAJRM/article/view/517 <p><strong>Background:</strong> Antimicrobial resistance among urinary <em>Escherichia coli</em> isolates can reduce the effectiveness of commonly used antibacterial agents.</p> <p><strong>Aims:</strong> This study examined antibiotic susceptibility and the presence of tetracycline-resistance genes in <em>E. coli</em> recovered from urine samples at Michael Okpara University of Agriculture, Umudike, Nigeria.</p> <p><strong>Methods:</strong> One hundred midstream urine samples were examined using cultural, morphological and biochemical methods. Six presumptive <em>E. coli</em> isolates were subjected to disc-diffusion susceptibility testing against ampicillin, ceftazidime, ciprofloxacin, gentamicin, amoxicillin–clavulanate, tetracycline and cefuroxime. Three tetracycline-resistant isolates were selected for 16S rRNA gene analysis and polymerase chain reaction screening for <em>tetA</em> and <em>tetB</em>.</p> <p><strong>Results:</strong> Six <em>E. coli</em> isolates were recovered, representing 6.0% of the samples examined. Three of the six isolates were resistant to tetracycline. Resistance to ampicillin, ciprofloxacin and amoxicillin–clavulanate was detected in two isolates each, while one isolate each was resistant to ceftazidime, gentamicin and cefuroxime. The three isolates selected for molecular analysis produced 16S rRNA amplicons. Among these three isolates, one carried both <em>tetA</em> and <em>tetB</em>, corresponding to 33.3% of the molecularly screened isolates and 16.7% of all six <em>E. coli</em> isolates.</p> <p><strong>Conclusion:</strong> Tetracycline resistance and associated efflux-mediated resistance genes were detected among a small number of urinary <em>E. coli</em> isolates. Larger studies using complete clinical data, validated molecular controls and broader resistance-gene panels are required to determine the local epidemiological significance of these findings.</p> Perfect Victory Nwakwuruibe Immaculata Ugochi Nwankwo Copyright (c) 2026 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2026-08-01 2026-08-01 20 8 1 11 10.9734/sajrm/2026/v20i8517