Open Access Original Research Article

Compatibility between Chemical Insecticides and Metarhizium anisopliae var. acridum

Solange Xavier-Santos, Bonifácio Peixoto Magalhães, Gesiane Ribeiro Guimarães, Wanderson Silva Dos Santos, Daniel Diego Costa Carvalho

South Asian Journal of Research in Microbiology, Page 1-7
DOI: 10.9734/sajrm/2021/v11i230245

The entomopathogenic fungus Metarhizium anisopliae var. acridum has been studied in different countries as mycoinsecticide against grasshoppers. One of the utilization strategies considered is the association between entomopathogenic fungi and sublethal dosages of chemical insecticides compatible with the biological control agent. The effect of different chemical insecticides at concentrations varying from 5 to 5000 ppm on the conidial germination and growth of the fungus M. anisopliae var. acridum (CG 423) was assessed for compatibility evalution. The best results with inhibitory concentration (IC50) on germination (liquid medium) and radial colonial growth (solid medium) of Metarhizium anisopliae var. acridum at 28°C was obtained with Teflubenzuron.

Open Access Original Research Article

Evaluation of the Bacteriological and Physicochemical Qualities of Some Brands of Bottled Water Sold in Owerri, Imo State

Kingsley Kelechi Onyekachi, Joy Nkeiruka Dike-Ndudim, Emeka Simon Anikwo, Chizaram Winners Ndubueze

South Asian Journal of Research in Microbiology, Page 8-17
DOI: 10.9734/sajrm/2021/v11i230246

This work was carried out in order to detect the presence of bacterial agent in the bottled water samples, and to evaluate the physicochemical qualities of these bottled water. Twenty samples selected from four different brands of bottled water sold in Owerri, were examined to determine their bacteriological and physicochemical qualities. Bacteriological analyses using Membrane filtration technique was carried out to determine the heterotrophic bacteria, total coliform and fecal coliform counts. Physicochemical qualities were also determined using standard methods. The heterotrophic bacterial count ranged from 0.00 to 12.00 CFU/ml, total coliform count ranged from 0.00 to 7.50 coliform/100ml. There was absence of fecal coliform in the samples. The pH, Color, Turbidity and Conductivity of the samples ranged from 5.18 to 7.28, 0.00 to 18.00 PCU, 0.55 to 1.62 NTU and 16.00 to 262.00 µs/cm respectively.  The Chloride, Iron and Nitrite content of the samples ranged from 16.99 to 27.98 mg/L, 0.01 to 0.07 mg/L Fe, and 0.00 to 0.34 mg/L respectively. The bacterial species isolated belong to the genera Staphylococcus, Proteus, Klebsiella, Bacillus and Escherichia. Quality wise, most of the water samples met the XYZ standard for bacteriological and physicochemical quality for drinking water with the exception of few. It can be deduced from this study, that none of the bottled water is suitable for drinking with regards to the bacteriological and physicochemical parameters tested. Though some samples passed the bacteriological examination, most of them were acidic and therefore not safe for consumption.